Full efficacy and long-term immunogenicity induced by the SARS-CoV-2 vaccine candidate MVA-CoV2-S in mice

被引：20

作者：

Lazaro-Frias, Adrian ^{[1
,2
]}

Perez, Patricia ^{[1
,2
]}

Zamora, Carmen ^{[1
]}

Sanchez-Cordon, Pedro J. ^{[3
]}

Guzman, Maria ^{[4
]}

Luczkowiak, Joanna ^{[5
]}

Delgado, Rafael ^{[5
,6
]}

Casasnovas, Jose M. ^{[4
]}

Esteban, Mariano ^{[1
]}

Garcia-Arriaza, Juan ^{[1
,2
]}

机构：

[1] CSIC, Ctr Nacl Biotecnol CNB, Dept Mol & Cellular Biol, Madrid 28049, Spain

[2] Ctr Invest Biomed Red Enfermedades Infecciosas CI, Madrid, Spain

[3] CSIC, Inst Nacl Invest & Tecnol Agr & Alimentaria INIA, Ctr Invest Sanidad Anim CISA, Pathol Dept, Madrid 28130, Spain

[4] CSIC, Ctr Nacl Biotecnol CNB, Dept Macromol Struct, Madrid 28049, Spain

[5] Inst Invest Hosp Univ 12 Octubre Imas1, Madrid, Spain

[6] Univ Complutense, Sch Med, Madrid 28040, Spain

来源：

NPJ VACCINES | 2022年 / 7卷 / 01期

关键词：

COVID-19; MVA;

D O I：

10.1038/s41541-022-00440-w

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Two doses of the MVA-CoV2-S vaccine candidate expressing the SARS-CoV-2 spike (S) protein protected K18-hACE2 transgenic mice from a lethal dose of SARS-CoV-2. This vaccination regimen prevented virus replication in the lungs, reduced lung pathology, and diminished levels of pro-inflammatory cytokines. High titers of IgG antibodies against S and receptor-binding domain (RBD) proteins and of neutralizing antibodies were induced against parental virus and variants of concern, markers that correlated with protection. Similar SARS-CoV-2-specific antibody responses were observed at prechallenge and postchallenge in the two-dose regimen, while the single-dose treatment does not avoid vaccine breakthrough infection. All vaccinated animals survived infection and were also protected to SARS-CoV-2 reinfection. Furthermore, two MVA-CoV2-S doses induced long-term memory S-specific humoral and cellular immune responses in C57BL/6 mice, 6 months after immunization. The efficacy and immunological benefits of the MVA-CoV2-S vaccine candidate against COVID-19 supports its consideration for human clinical trials.

引用

页数：15

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