Determination of molar masses of macromolecules by size exclusion chromatography-light scattering not requiring knowledge of refractive index increments

被引：7

作者：

Lavric, Simona ^{[2
]}

Preis, Jasmin ^{[1
]}

Rosenauer, Christine ^{[3
]}

Radke, Wolfgang ^{[1
]}

机构：

[1] PSS Polymer Stand Serv GmbH, Mainz, Germany

[2] Melamin Dd, Kocevje, Slovenia

[3] Max Planck Inst Polymer Res, Mainz, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2017年 / 1520卷

关键词：

SEC-light scattering; GPC-light scattering; Calibration; Refractive index increment; dn/dc; GEL-PERMEATION CHROMATOGRAPHY; MOLECULAR-WEIGHTS; RAYLEIGH RATIO; PURE LIQUIDS; COPOLYMERS; LASER;

D O I：

10.1016/j.chroma.2017.09.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new approach for the calibration of SEC-light scattering (SEC-LS) setups is proposed, which requires solely the molar mass of a reference polymer. Neither the specific refractive index increment of the calibrant nor of the analyte is required. Comparison of the molar masses derived in different solvents for a large number of chemically different polymers shows that the new approach yields the same molar masses as if molar masses were derived using dn/dc to calibrate the light scattering setup. The approach therefore allows easier determination of molar masses by SEC-LS. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：103 / 106

页数：4

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