pH-Sensitive Mechanical Properties of Elastin-Based Hydrogels

被引:20
作者
Hollingshead, Sydney [1 ]
Liu, Julie C. [1 ,2 ]
机构
[1] Purdue Univ, Davidson Sch Chem Engn, W Lafayette, IN 47907 USA
[2] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
基金
美国国家科学基金会;
关键词
elastomeric materials; environmentally responsive materials; ionizable amino acids; recombinant proteins; smart materials; RESPONSIVE POLYMERS; PROTEIN; CELL; MATRIX; POLYPENTAPEPTIDE; BIOCOMPATIBILITY; PURIFICATION; TROPOELASTIN; ACETYLATION; TRANSITIONS;
D O I
10.1002/mabi.201900369
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ionizable amino acids in protein-based hydrogels can confer pH-responsive behavior. Because elastin-like polypeptides (ELPs) have an established sequence and can crosslink to form hydrogels, they are an ideal system for creating pH-sensitive materials. This study examines different parameters that might affect pH-sensitive behavior and characterizes the mechanical and physical properties between pH 3 and 11 of three ELP-based crosslinked hydrogels. The first finding is that varying the amount of crosslinker affects the overall stiffness and resilience of the hydrogels but does not strongly affect water content, swelling ratio, or pH sensitivity. Second, the choice of two popular tag sequences, which vary in histidine and aspartic acid content, does not have a strong effect on pH-sensitive properties. Last, selectively blocking lysine and tyrosine residues through acetylation significantly decreases the pH-sensitive zeta potential. Acetylated hydrogels also demonstrate different behavior at low pH values with reduced swelling, reduced water content, and higher stiffness. Overall, this work demonstrates that ELP hydrogels with ionizable groups are promising materials for environmentally-responsive applications such as drug delivery, tissue engineering, and microfluidics.
引用
收藏
页数:12
相关论文
共 50 条
  • [21] Thermoresponsive self-assembled elastin-based nanoparticles for delivery of BMPs
    Bessa, Paulo C.
    Machado, Raul
    Nuernberger, Sylvia
    Dopler, Daniela
    Banerjee, Asmita
    Cunha, Antonio M.
    Carlos Rodriguez-Cabello, J.
    Redl, Heinz
    van Griensven, Martijn
    Reis, Rui L.
    Casal, Margarida
    JOURNAL OF CONTROLLED RELEASE, 2010, 142 (03) : 312 - 318
  • [22] Preparation and recovery performance of modified cellulase with pH-sensitive separation properties
    Zhang, Huihui
    Wang, Shuang
    Yao, Yao
    Qi, Kehui
    Yu, Chunyan
    Liu, Qing
    Li, Guorui
    Lu, Yanju
    Li, Lu
    Zhou, Jierui
    INDUSTRIAL CROPS AND PRODUCTS, 2024, 222
  • [23] A pH-sensitive regenerated cellulose membrane
    Xiong, Xiaopeng
    Duan, Jiangjiang
    Zou, Wewei
    He, Xumin
    Zheng, Wei
    JOURNAL OF MEMBRANE SCIENCE, 2010, 363 (1-2) : 96 - 102
  • [24] pH-sensitive polymers for drug delivery
    Huh, Kang Moo
    Kang, Han Chang
    Lee, Young Ju
    Bae, You Han
    MACROMOLECULAR RESEARCH, 2012, 20 (03) : 224 - 233
  • [25] Structure formation in pH-sensitive hydrogels composed of sodium caseinate and N,O-carboxymethyl chitosan
    Wei, Yanxia
    Xie, Rui
    Lin, Yanbin
    Xu, Yunfei
    Wang, Fengxia
    Liang, Wanfu
    Zhang, Ji
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2016, 89 : 353 - 359
  • [26] Development of pH-sensitive and antibacterial gelatin/citric acid/Ag nanocomposite hydrogels with potential for biomedical applications
    Shafagh, Nima
    Sabzi, Mohammad
    Afshari, Mohammad Javad
    JOURNAL OF POLYMER RESEARCH, 2018, 25 (12)
  • [27] Dextran derivative-based pH-sensitive liposomes for cancer immunotherapy
    Yuba, Eiji
    Tajima, Naoki
    Yoshizaki, Yuta
    Harada, Atsushi
    Hayashi, Hiroshi
    Kono, Kenji
    BIOMATERIALS, 2014, 35 (09) : 3091 - 3101
  • [28] pH-sensitive nanostructured architectures based on synthetic and/or natural weak polyelectrolytes
    Mihai, Marcela
    Stoica, Iuliana
    Schwarz, Simona
    COLLOID AND POLYMER SCIENCE, 2011, 289 (12) : 1387 - 1396
  • [29] Application of elastin-based nanoparticles displaying antibody binding domains for a homogeneous immunoassay
    Sugihara, Tsutomu
    Mie, Masayasu
    Kobatake, Eiry
    ANALYTICAL BIOCHEMISTRY, 2018, 544 : 72 - 79
  • [30] Bioinspired pH-sensitive riboflavin controlled-release alkaline hydrogels based on blue crab chitosan: Study of the effect of polymer characteristics
    Hamdi, Marwa
    Nasri, Rim
    Li, Suming
    Nasri, Moncef
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 152 : 1252 - 1264