Respiratory Syncytial Virus and Other Viral Infections among Children under Two Years Old in Southern Vietnam 2009-2010: Clinical Characteristics and Disease Severity

被引：26

作者：

Lien Anh Ha Do ^{[1
,2
]}

Juliet E. Bryant ^{[1
,3
]}

Anh Tuan Tran ^{[4
]}

Bach Hue Nguyen ^{[4
]}

Thi Thu Loan Tran ^{[5
]}

Quynh Huong Tran ^{[5
]}

Quoc Bao Vo ^{[5
]}

Nguyen Anh Tran Dac ^{[5
]}

Hong Nhien Trinh ^{[4
]}

Thi Thanh Hai Nguyen ^{[4
]}

Bao Tinh Le Binh ^{[4
]}

Khanh Le ^{[4
]}

Minh Tien Nguyen ^{[4
]}

Quang Tung Thai ^{[4
]}

Thanh Vu Vo ^{[4
]}

Ngoc Quang Minh Ngo ^{[4
]}

Thi Kim Huyen Dang ^{[5
]}

Ngoc Huong Cao ^{[5
]}

Thu Van Tran ^{[5
]}

Lu Viet Ho ^{[5
]}

Farrar, Jeremy ^{[1
]}

Menno de Jong ^{[1
,3
,6
]}

Rogier van Doorn, H. ^{[1
,3
]}

机构：

[1] Univ Oxford, Clin Res Unit, Wellcome Trust Major Overseas Program, Ho Chi Minh City, Vietnam

[2] Murdoch Childrens Res Inst, Melbourne, Vic, Australia

[3] Univ Oxford, Nuffield Dept Clin Med, Oxford, England

[4] Children Hosp 1, 341 Su Van Hanh,Ward 10,Dist 10, Ho Chi Minh City, Vietnam

[5] Children Hosp 2, 14 Ly Tu Trong,Dist 1, Ho Chi Minh City, Vietnam

[6] Univ Amsterdam, Acad Med Ctr, Dept Med Microbiol, Amsterdam, Netherlands

来源：

PLOS ONE | 2016年 / 11卷 / 08期

基金：

英国惠康基金;

关键词：

TRACT INFECTIONS; ACUTE BRONCHIOLITIS; HOSPITALIZED INFANTS; PCR ASSAY; LOAD; RHINOVIRUS; COMMUNITY; COINFECTION; EPIDEMIOLOGY; ASSOCIATION;

D O I：

10.1371/journal.pone.0160606

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background Despite a high burden of respiratory syncytial virus (RSV) infections among children, data on demographic and clinical characteristics of RSV are scarce in low and middle income countries. This study aims to describe the viral etiologies, the demographic, epidemiological, and clinical characteristics of children under two years of age who were hospitalized with a lower respiratory tract infections (LRTI), focusing on RSV (prevalence, seasonality, subgroups, viral load) and its association with disease severity. Methods A prospective study among children under two years of age, hospitalized with LRTI was conducted in two referral pediatric hospitals in Ho Chi Minh City, Vietnam, from May 2009 to December 2010. Socio-demographic, clinical data and nasopharyngeal swabs were collected on enrolment and discharge. Multiplex real-time RT-PCR (13 viruses) and quantitative RSV RT-PCR were used to identify viral pathogens, RSV load and subgroups. Results Among 632 cases, 48% were RSV positive. RSV infections occurred at younger age than three other leading viral infections i.e rhinovirus (RV), metapneumovirus (MPV), parainfluenza virus (PIV-3) and were significantly more frequent in the first 6 months of life. Clinical severity score of RSV infection was significantly higher than PIV-3 but not for RV or MPV. In multivariate analysis, RV infection was significantly associated with severity while RSV infection was not. Among RSV infections, neither viral load nor viral co-infections were significantly associated with severity. Young age and having fever at admission were significantly associated with both RSV and LRTI severity. A shift in RSV subgroup predominance was observed during two consecutive rainy seasons but was not associated with severity. Conclusion We report etiologies, the epidemiological and clinical characteristics of LRTI among hospitalized children under two years of age and risk factors of RSV and LRTI severity.

引用

页数：16

共 52 条

[21] Relationships among specific viral pathogens, virus-induced interleukin-8, and respiratory symptoms in infancy [J].

Gern, JE ;

Martin, MS ;

Anklam, KA ;

Shen, K ;

Roberg, KA ;

Carlson-Dakes, KT ;

Adler, K ;

Gilbertson-White, S ;

Hamilton, R ;

Shult, PA ;

Kirk, CJ ;

Da Silva, DF ;

Sund, SA ;

Kosorok, MR ;

Lemanske, RF .

PEDIATRIC ALLERGY AND IMMUNOLOGY, 2002, 13 (06) :386-393

[22] Correlation of viral load as determined by real-time RT-PCR and clinical characteristics of respiratory syncytial virus lower respiratory tract infections in early infancy [J].

Gerna, G. ;

Campanini, G. ;

Rognoni, V. ;

Marchi, A. ;

Rovida, F. ;

Piralla, A. ;

Percivalle, E. .

JOURNAL OF CLINICAL VIROLOGY, 2008, 41 (01) :49-52

[23] Correlation of Rhinovirus Load in the Respiratory Tract and Clinical Symptoms in Hospitalized Immunocompetent and Immunocompromised Patients [J].

Gerna, G. ;

Piralla, A. ;

Rovida, F. ;

Rognoni, V. ;

Marchi, A. ;

Locatelli, F. ;

Meloni, F. .

JOURNAL OF MEDICAL VIROLOGY, 2009, 81 (08) :1498-1507

[24]

HALL CB, 1976, J PEDIATR-US, V89, P11, DOI 10.1016/S0022-3476(76)80918-3

[25] Disease Severity and Viral Load Are Correlated in Infants With Primary Respiratory Syncytial Virus Infection in the Community [J].

Houben, M. L. ;

Coenjaerts, F. E. J. ;

Rossen, J. W. A. ;

Belderbos, M. E. ;

Hofland, R. W. ;

Kimpen, J. L. L. ;

Bont, L. .

JOURNAL OF MEDICAL VIROLOGY, 2010, 82 (07) :1266-1271

[26] Development and evaluation of a four-tube real time multiplex PCR assay covering fourteen respiratory viruses, and comparison to its corresponding single target counterparts [J].

Jansen, Rogier R. ;

Schinkel, Janke ;

Koekkoek, Sylvie ;

Pajkrt, Dasja ;

Beld, Marcel ;

de Jong, Menno D. ;

Molenkamp, Richard .

JOURNAL OF CLINICAL VIROLOGY, 2011, 51 (03) :179-185

[27] Evaluation of quantitative and type-specific real-time RT-PCR assays for detection of respiratory syncytial virus in respiratory specimens from children [J].

Kuypers, J ;

Wright, N ;

Morrow, R .

JOURNAL OF CLINICAL VIROLOGY, 2004, 31 (02) :123-129

[28]

Lee Jian-Te, 2007, Journal of Microbiology Immunology and Infection, V40, P293

[29] A sensitive real-time PCR for detection and subgrouping of human respiratory syncytial virus [J].

Lien Anh Ha Do ;

van Doorn, H. Rogier ;

Bryant, Juliet E. ;

My Ngoc Nghiem ;

Vinh Chau Nguyen Van ;

Cong Khanh Vo ;

Minh Dung Nguyen ;

Tinh Hien Tran ;

Farrar, Jeremy ;

de Jong, Menno D. .

JOURNAL OF VIROLOGICAL METHODS, 2012, 179 (01) :250-255

[30] RHINOVIRUS ASSOCIATED WITH SEVERE LOWER RESPIRATORY TRACT INFECTIONS IN CHILDREN [J].

Louie, Janice K. ;

Roy-Burman, Arup ;

Guardia-LaBar, Lilly ;

Boston, Erica J. ;

Kiang, David ;

Padilla, Tasha ;

Yagi, Shigeo ;

Messenger, Sharon ;

Petru, Ann M. ;

Glaser, Carol A. ;

Schnurr, David P. .

PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2009, 28 (04) :337-339

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