Propidium ion enters viable cells with high membrane potential during live-dead staining

被引：70

作者：

Kirchhoff, Christian ^{[1
]}

Cypionka, Heribert ^{[1
]}

机构：

[1] Carl von Ossietzky Univ Oldenburg, Inst Chem & Biol Marine Environm ICBM, Oldenburg, Germany

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2017年 / 142卷

关键词：

Dinoroseobacter shibae; Bacillus subtilis; Sybr Green I; DiOC(2)(3); Propidium iodide; Fluorescence microscopy; DINOROSEOBACTER-SHIBAE; ANOXIA;

D O I：

10.1016/j.mimet.2017.09.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Live-dead staining with propidium iodide can give erroneous results for bacteria showing high membrane potentials. We observed uptake of propidium ions across intact cell membranes for Dinoroseobacter shibae and Bacillus subtilis. Apparently, a high membrane potential facilitates breakthrough of the double-charged propidium ion and can mark viable cells as dead.

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页码：79 / 82

页数：4

相关论文

共 9 条

[1] Dinoroseobacter shibae gen. nov., sp nov., a new aerobic phototrophic bacterium isolated from dinoflagellates [J].

Biebl, H ;

Allgaier, M ;

Tindall, BJ ;

Koblizek, M ;

Lünsdorf, H ;

Pukall, R ;

Wagner-Döbler, I .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2005, 55 :1089-1096

[2] LIVE/DEAD® BacLight™:: application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water [J].

Boulos, L ;

Prévost, M ;

Barbeau, B ;

Coallier, J ;

Desjardins, R .

JOURNAL OF MICROBIOLOGICAL METHODS, 1999, 37 (01) :77-86

[3]

Cypionka H., 2016, BIOSPEKTRUM, V22, P143, DOI [10.1007/s12268-016-0668-1, DOI 10.1007/S12268-016-0668-1]

[4] Red but not dead? Membranes of stressed Saccharomyces cerevisiae are permeable to propidium iodide [J].

Davey, H. M. ;

Hexley, P. .

ENVIRONMENTAL MICROBIOLOGY, 2011, 13 (01) :163-171

[5] Influence of light and anoxia on chemiosmotic energy conservation in Dinoroseobacter shibae [J].

Holert, Johannes ;

Hahnke, Sarah ;

Cypionka, Heribert .

ENVIRONMENTAL MICROBIOLOGY REPORTS, 2011, 3 (01) :136-141

[6] Boosted Membrane Potential as Bioenergetic Response to Anoxia in Dinoroseobacter shibae [J].

Kirchhoff, Christian ;

Cypionka, Heribert .

FRONTIERS IN MICROBIOLOGY, 2017, 8

[7] Application of flow cytometry to segregated kinetic modeling based on the physiological states of microorganisms [J].

Quiros, Covadonga ;

Herrero, Monica ;

Garcia, Luis A. ;

Diaz, Mario .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (12) :3993-4000

[8] Light Enhances Survival of Dinoroseobacter shibae during Long-Term Starvation [J].

Soora, Maya ;

Cypionka, Heribert .

PLOS ONE, 2013, 8 (12)

[9] Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications [J].

Zipper, H ;

Brunner, H ;

Bernhagen, J ;

Vitzthum, F .

NUCLEIC ACIDS RESEARCH, 2004, 32 (12)