Polymer-based elemental tags for sensitive Bioassays

被引：242

作者：

Lou, Xudong

Zhang, Guohua

Herrera, Isaac

Kinach, Robert

Ornatsky, Olga

Baranov, Vladimir

Nitz, Mark

Winnik, Mitchell A.

机构：

[1] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON M5S 3G9, Canada

[2] Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 32期

关键词：

antibodies; bioassays; chelates; mass spectrometry; polymers;

D O I：

10.1002/anie.200700796

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) It's elemental: A water-soluble polymer bearing multiple metal-chelating ligands has been used as a tag for bioassays with inductively coupled plasma mass spectrometry. The tag was covalently conjugated to antibodies, and the polymer-antibody constructs were loaded with lanthanide ions (Ln3+) and used for the simultaneous assay of five orthogonally labeled antibodies against cell surface antigens that differ in abundance by more than two orders of magnitude. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：6111 / 6114

页数：4

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