Label-Free Transient Absorption Microscopy for Red Blood Cell Flow Velocity Measurement in Vivo

被引：10
作者：
Chen, Tao [1 ,2 ]
Huang, Yanyi [1 ,2 ,3 ]
机构：
[1] Peking Univ, Biodynam Opt Imaging Ctr BIOPIC, Beijing 100871, Peoples R China
[2] Peking Univ, Coll Engn, Beijing 100871, Peoples R China
[3] Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing Adv Innovat Ctr Genom ICG, Beijing 100871, Peoples R China
来源：
ANALYTICAL CHEMISTRY | 2017年 / 89卷 / 19期
基金：
中国国家自然科学基金;
关键词：
CIRCULATING TUMOR-CELLS; LIVE CELLS; ZEBRAFISH; TOMOGRAPHY; DISEASE;
D O I：
10.1021/acs.analchem.7b01952
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Red blood cells have intrinsic transient absorption property at the near-infrared region, allowing for label-free detection and imaging. We present a new approach to measure the blood flow velocity in vivo with a transient absorption microscope and correlation analyses of signal sequences. With specific scan modes, we have quantitatively obtained the flow velocity in capillaries, arteries, and veins in a live zebrafish with accuracy of about 30 mu m/s. In addition, a high-resolution three-dimensional vessel network can be reconstructed through this approach with spatial resolution of 1 mu m.
引用
收藏
页码：10120 / 10123
页数：4
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