Hematocrit and standardization in DBS analysis: A practical approach for hormones mainly present in the plasma fraction

被引：24
作者：
Ackermans, Mariette T. [1 ]
de Kleijne, Vera [1 ]
Martens, Frans [1 ,2 ]
Heijboer, Annemieke C. [2 ]
机构：
[1] Univ Amsterdam, Amsterdam UMC, Dept Clin Chem, Endocrine Lab, Amsterdam, Netherlands
[2] Vrije Univ Amsterdam, Amsterdam UMC, Dept Clin Chem, Endocrine Lab, Amsterdam, Netherlands
来源：
CLINICA CHIMICA ACTA | 2021年 / 520卷
关键词：
DRIED BLOOD SPOTS; QUANTITATIVE-ANALYSIS; BIAS; QUANTIFICATION; BIOANALYSIS; PREDICTION; MATRIX;
D O I：
10.1016/j.cca.2021.06.014
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Background and aim: Dried blood spot (DBS) sampling has many advantages over conventionally used blood samples, but is thought to suffer from hematocrit related issues. The aim of our research was to investigate whether reliable results can be obtained without bothering about hematocrit effects in DBS analysis of analytes that are mainly present in the plasma compartment. Materials and Methods: Venous blood samples with variation in hematocrit and spotted volume were prepared. Spot diameter and 25-OH Vitamin D3 and testosterone concentrations were measured. Moreover, DBS and plasma concentrations of 25-OH Vitamin D3, testosterone and hematocrit were determined in random patient samples. Results: DBS spot size was linearly related to hematocrit. Measured DBS concentrations of 25-OH Vitamin D3 and testosterone were independent of hematocrit and spotted volume. Determining the relation between plasma and DBS concentration resulted in a factor that can be used to convert DBS concentrations to standardized plasma concentrations. Conclusion: Addressing the hematocrit issue is not necessary for hormones that are mainly present in the plasma compartment. The relation between plasma and DBS concentration can be used to convert DBS concentrations to standardized plasma concentrations which makes interpretation of DBS concentrations easier.
引用
收藏
页码：179 / 185
页数：7
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