Detection of 81 of 81 known mouse beta-globin promoter mutations with T4 endonuclease VII - The EMC method

被引：47

作者：

Youil, R ^{[1
]}

Kemper, B ^{[1
]}

Cotton, RGH ^{[1
]}

机构：

[1] UNIV COLOGNE,INST GENET,D-50674 COLOGNE,GERMANY

来源：

GENOMICS | 1996年 / 32卷 / 03期

关键词：

D O I：

10.1006/geno.1996.0138

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The enzyme mismatch cleavage (EMC) method relies on the use of the resolvase T4 Endonuclease VII to cleave and thus detect mismatches in heteroduplex DNA formed by annealing normal DNA with mutant DNA. Detection is based on cleavage 3' to the mismatch within a few nucleotides. We report the detection of all 81 different homozygous single-basepair changes tested and present in the mouse beta-globin promoter by using the EMC method with a single set of conditions, Efficiency of cleavage was rated as strong, medium, or weak based on the intensity of the cleavage product(s) compared with background bands on autoradiography. We expect this method to detect near 100% of mutations. (C) 1996 Academic Press, Inc.

引用

页码：431 / 435

页数：5

共 10 条

[1] Improved strategy for mutation detection - A modification to the enzyme mismatch cleavage method [J].