An In Vitro Model for Identifying Cardiac Side Effects of Anesthetics

被引：9

作者：

Chang, Alex C. Y. ^{[1
,2
,3
]}

Chang, Andrew C. H. ^{[1
,2
]}

Nicin, Luka ^{[1
]}

Weber, Gerhard J. ^{[4
]}

Holbrook, Colin ^{[1
]}

Davies, M. Frances ^{[5
,6
]}

Blau, Helen M. ^{[1
,2
]}

Bertaccini, Edward J. ^{[5
,6
]}

机构：

[1] Stanford Univ, Baxter Lab Stem Cell Biol, Dept Microbiol & Immunol, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA

[2] Stanford Univ, Stanford Cardiovasc Inst, Sch Med, Stanford, CA 94305 USA

[3] Stanford Univ, Div Cardiovasc Med, Sch Med, Stanford, CA 94305 USA

[4] Univ Washington, Div Cardiol, Seattle, WA 98195 USA

[5] Stanford Univ, Sch Med, Dept Anesthesia, Stanford, CA 94305 USA

[6] Palo Alto Vet Affairs Hlth Care Syst, Palo Alto, CA USA

来源：

ANESTHESIA AND ANALGESIA | 2020年 / 130卷 / 01期

基金：

美国国家卫生研究院; 加拿大健康研究院;

关键词：

PLURIPOTENT; CONTRACTILITY;

D O I：

10.1213/ANE.0000000000003757

中图分类号：

R614 [麻醉学];

学科分类号：

100217 ;

摘要：

The understanding of anesthetic side effects on the heart has been hindered by the lack of sophisticated clinical models. Using micropatterned human-induced pluripotent stem cell-derived cardiomyocytes, we obtained cardiac muscle depressant profiles for propofol, etomidate, and our newly identified anesthetic compound KSEB01-S2. Propofol was the strongest depressant among the 3 compounds tested, exhibiting the largest decrease in contraction velocity, depression rate, and beating frequency. Interestingly, KSEB01-S2 behaved similarly to etomidate, suggesting a better cardiac safety profile. Our results provide a proof-of-concept for using human-induced pluripotent stem cell-derived cardiomyocytes as an in vitro platform for future drug design.

引用

页码：E1 / E4

页数：4

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