QF-PCR: application, overview and review of the literature

被引：49

作者：

Mann, Kathy ^{[1
]}

Ogilvie, Caroline Mackie ^{[2
]}

机构：

[1] Guys & St Thomas Hosp, GSTS Pathol, Dept Cytogenet, London SE1 9RT, England

[2] Guys & St Thomas NH Fdn Trust, Dept Cytogenet, London SE1 9RT, England

来源：

PRENATAL DIAGNOSIS | 2012年 / 32卷 / 04期

关键词：

QF-PCR; PGD; karyotyping; RAPID PRENATAL-DIAGNOSIS; POLYMERASE-CHAIN-REACTION; QUANTITATIVE FLUORESCENT PCR; COMMON CHROMOSOME ANEUPLOIDIES; LONG-TERM CULTURE; DOWNS-SYNDROME; UNCULTURED AMNIOCYTES; KARYOTYPE ANALYSIS; COMPLETE DISCREPANCY; FULL KARYOTYPE;

D O I：

10.1002/pd.2945

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Quantitative fluorescent polymerase chain reaction has been in diagnostic use in the UK for over 10 years and has proved to be a cost-effective, robust and accurate rapid prenatal test for common aneuploidies. Specific advantages include detection of triploidy, mosaicism and maternal cell contamination. Its application at our centre is described, with developments including stand-alone testing and improvements in strategies for the preparation and testing of chorionic villus biopsies. (C) 2012 John Wiley & Sons, Ltd.

引用

页码：309 / 314

页数：6

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