An electrochemical investigation of glucose oxidase at a CdS nanoparticles modified electrode

被引：188

作者：

Huang, YX ^{[1
]}

Zhang, WJ ^{[1
]}

Xiao, H ^{[1
]}

Li, GX ^{[1
]}

机构：

[1] Nanjing Univ, Dept Biochem, Natl Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2005年 / 21卷 / 05期

基金：

中国国家自然科学基金;

关键词：

glucose oxidase; CdS nanoparticles; electron-transfer; enzymatic activity; biosensor;

D O I：

10.1016/j.bios.2005.01.012

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The direct electrochemistry of glucose oxidase (GOD) adsorbed on a US nanoparticles modified pyrolytic graphite electrode was investigated, where the enzyme demonstrated significantly enhanced electron-transfer reactivity. GOD adsorbed on CdS nanoparticles maintained its bioactivity and structure, and could electrocatalyze the reduction of dissolved oxygen, which resulted in a great increase of the reduction peak current. Upon the addition of glucose, the reduction peak current decreased, which could be used for glucose detection. Performance and characteristics of the fabricated glucose biosensor were assessed with respect to detection limit, sensitivity, storage stability and interference exclusion. The results showed that the fabricated biosensor was sensitive and stable in detecting glucose, indicating that CdS nanoparticle was a good candidate material for the immobilization of enzyme in glucose biosensor construction. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：817 / 821

页数：5

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