Development of Colloidal Gold Lateral Flow Immunoassay for Quantitative Detection of Florfenicol

被引：3

作者：

Han Jiao-Jiao ^{[1
]}

Hu Li-Ming ^{[1
]}

Yi Yang ^{[1
]}

Liu Miao ^{[1
]}

Xia Jun ^{[2
]}

Xu Guo-Mao ^{[2
]}

Luo Kai ^{[1
]}

Wang Qi ^{[1
]}

Lai Wei-Hua ^{[1
]}

机构：

[1] State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China

[2] Jiangxi Inst Vet Drug & Feedstuff Control, Nanchang 330047, Jiangxi, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2017年 / 45卷 / 08期

关键词：

Colloidal gold; Florfenicol; Lateral flow immunoassay; Quantitative detection; Strip; AMINE; THIAMPHENICOL; ELISA; CHLORAMPHENICOL; OXYTETRACYCLINE; ESTABLISHMENT; RESIDUES; MYKISS; STRIP; FOOD;

D O I：

10.11895/j.issn.0253-3820.170207

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for quantitative detection of florfenicol by colloidal gold lateral flow immunoassay was developed. The experimental conditions including pH value, concentrations of antibody in the process of conjugation between the colloidal gold and antibody, amount of gold. labeled antibody, concentration of the antigen sprayed on test lines (T line), and detection time were optimized. With a colloidal gold strip reader, the signal intensity of T lines and control lines (C line) on lateral flow strips was recorded. The T/C ratio of negative control and positive samples was defined as B-0 and B-x, and the standard curve was established by plotting the B-x/B-0 ratio against the concentration of florfenicol. This assay showed a good linear range from 0. 1 to 1. 5 ng/mL with the limit of detection of 0. 08 ng/mL, while the result could be obtained within 15 min. The result showed that this quantitative method was convenient and rapid, and could be used in screening a large amount of samples on site.

引用

页码：1188 / 1194

页数：7

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