Anti-Herpes Simplex Virus Type 2 of Drymaria diandra Blume Medicinal Plant

被引:0
作者
Nikomtat, Jiraporn [1 ,2 ]
Thongwai, Narumol [2 ]
Lumyong, Saisamorn [2 ]
Tragoolpua, Yingmanee [2 ]
机构
[1] Chiang Mai Univ, Program Biotechnol, Grad Sch, Chiang Mai 50200, Thailand
[2] Chiang Mai Univ, Dept Biol, Fac Sci, Chiang Mai 50200, Thailand
来源
CHIANG MAI JOURNAL OF SCIENCE | 2011年 / 38卷 / 03期
关键词
Drymaria diandra Blume; herpes simplex virus; anti-viral; plant; PROTEINS; CELLS; REPLICATION; HEPARIN; BINDING; CAPSIDS;
D O I
暂无
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Anti-herpes simplex virus type 2 of dichloromethane and methanol extracts of Thai medicinal plant, Drymaria diandra Blume was investigated on Green Monkey Kidney cells. Cytotoxicity tests performed by the MTT assay revealed that 50% cytotoxicity dose of dichloromethane and methanol extracts were 90 +/- 2 and 631 +/- 32 mu g/ml respectively. In all experiments, non-toxic concentrations of the plant extracts were used to test the inhibitory effect on various steps of herpes simplex virus type 2 (HSV-2) multiplication cycle. HSV-2 particles were directly inactivated by the extracts since 1 hour of treatment. HSV-2 infection was inhibited by the methanol extract with the 50% effective dose of 166.6 +/- 23.1 mu g/ml. Moreover, both extracts inhibited HSV-2 attachment and penetration. The extracts also showed potent inhibitory activities against viral deoxyribonucleic acid synthesis as determined by real time polymerase chain reaction. Western blot analysis showed that HSV-2 proteins, particularly those at approximately 45 kDa were inhibited by the extracts after detection by horseradish peroxidase-conjugated immunoglobulin G against HSV. Therefore, this study demonstrated the inhibitory efficacy of Drymaria diandra Blume extracts against herpes simplex virus infection as a potential anti-HSV agent.
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页码:439 / 452
页数:14
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