Actin enhances the activation of human neutrophil NADPH oxidase in a cell-free system

被引：40

作者：

Morimatsu, T ^{[1
]}

Kawagoshi, A ^{[1
]}

Yoshida, K ^{[1
]}

Tamura, M ^{[1
]}

机构：

[1] EHIME UNIV,FAC ENGN,DEPT APPL BIOCHEM,MATSUYAMA,EHIME 790,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 230卷 / 01期

关键词：

D O I：

10.1006/bbrc.1996.5881

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cell-free activation of human neutrophil NADPH oxidase (O-2(-) generating enzyme) was enhanced by exogenously added G-actin (actin monomer). When cytosol, a constituent of the system, was pretreated with DNase I, which may bind to G-actin (endogenous) to block polymerization, the activation of NADPH oxidase was significantly suppressed. The activation was also impaired when cytosol G-actin was removed by DNase I-linked resin, being completely restored by the addition of G-actin. These results suggest a role of actin and its polymerization in the activation of NADPH oxidase of human neutrophils. (C) 1997 Academic Press

引用

页码：206 / 210

页数：5

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