Oxygen effect on Dehalococcoides viability and biomarker quantification

被引:95
作者
Amos, Benjamin K. [1 ]
Ritalahti, Kirsti M. [1 ]
Cruz-Garcia, Claribel [1 ]
Padilla-Crespo, Elizabeth [2 ]
Loeffler, Frank E. [1 ,2 ]
机构
[1] Georgia Inst Technol, Sch Civil & Environm Engn, Atlanta, GA 30332 USA
[2] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA
关键词
D O I
10.1021/es703227g
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Oxygen-sensitive Dehalococcoides bacteria play crucial roles in detoxification of chlorinated contaminants (e.g., chlorinated ethenes), and bioremediation monitoring relies on quantification of Dehalococcoides DNA and RNA biomarkers. To explore the effects of oxygen on Dehalococcoides activity, viability, and biomarker quantification, batch experiments with a tetrachloroethene-to-ethene dechlorinating consortium (Bio-Dechlor INOCULUM [BDI]) harboring multiple Dehalococcoides strains were performed to quantify the effects of <= 4 mg/L dissolved oxygen. Oxygen inhibited reductive dechlorination, and only incomplete dechlorination to vinyl chloride (VC) occurred following oxygen consumption and extended incubation periods (89 days). Following 30 days of oxygen exposure and subsequent oxygen removal (i.e., reversibility experiments), all trichloroethene- (TCE-) fed cultures dechlorinated TCE to VC, but VC dechlorination to ethene occurred in only one out of fourteen replicates. These results suggest that Dehalococcoides strains respond differently to oxygen exposure, and strains catalyzing the VC-to-ethene dechlorination step are more susceptible to oxygen inhibition. Quantitative real-time PCR (qPCR) analysis detected a 1-1.5 order-of-magnitude decrease in the number of Dehalococcoides biomarker genes (i.e., 16S rRNA gene and the reductive dehalogenase [RDase] genes tceA, vcrA, bvcA) in the oxygen-amended cultures, but qPCR analysis failed to distinguish viable, dechlorinating from irreversibly inhibited (nonviable) Dehalococcoides cells. Reverse transcriptase qPCR (RT-qPCR) detected Dehalococcoides gene transcripts in the oxygen-amended, non-dechlorinating cultures, and biomarker transcription did not always correlate with dechlorination (in)activity. Enhanced molecular tools that complement existing protocols and provide quantitative information on the viability and activity of the Dehalococcoides population are desirable.
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收藏
页码:5718 / 5726
页数:9
相关论文
共 47 条
  • [1] Growth of Dehalococcoides strains with chlorophenols as electron acceptors
    Adrian, Lorenz
    Hansen, Sigrid K.
    Fung, Jennifer M.
    Goerisch, Helmut
    Zinder, Stephen H.
    [J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2007, 41 (07) : 2318 - 2323
  • [2] AIELLO MR, 2003, THESIS MICHIGAN STAT
  • [3] Effects of the nonionic surfactant tween 80 on microbial reductive dechlorination of chlorinated ethenes
    Amos, Benjamin K.
    Daprato, Rebecca C.
    Hughes, Joseph B.
    Pennell, Kurt D.
    Loffler, Frank E.
    [J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2007, 41 (05) : 1710 - 1716
  • [4] Experimental evaluation and mathematical modeling of microbially enhanced tetrachloroethene (PCE) dissolution
    Amos, Benjamin K.
    Christ, John A.
    Abriola, Linda M.
    Pennell, Kurt D.
    Loffler, Frank E.
    [J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2007, 41 (03) : 963 - 970
  • [5] Bradley Paul M., 2003, Bioremediation Journal, V7, P81, DOI 10.1080/713607980
  • [6] Costilow R. N, 1981, MANUAL METHODS GENER, P66
  • [7] Growth of a Dehalococcoides-like microorganism on vinyl chloride and cis-dichloroethene as electron acceptors as determined by competitive PCR
    Cupples, AM
    Spormann, AM
    McCarty, PL
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (02) : 953 - 959
  • [8] Bioaugmentation for accelerated in situ anaerobic bioremediation
    Ellis, DE
    Lutz, EJ
    Odom, JM
    Buchanan, RJ
    Bartlett, CL
    Lee, MD
    Harkness, MR
    Deweerd, KA
    [J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2000, 34 (11) : 2254 - 2260
  • [9] FLETCHER KE, 2007, SERDP ESTCP PARTN EN
  • [10] *GEOS, 2005, BIOAUGM REM CHLOR SO