Design of Ligands-Conjuncted Lipid Nanobubbles as Ultrasound Contrast Agents Targeted to Atherosclerotic Plaques

被引：7
作者：
Wang, Kunkun [1 ]
Guo, Binbin [1 ]
Zhang, Feng [1 ]
Wang, Xiaowei [1 ]
Yang, Jiemei [1 ]
Zheng, Yaru [1 ]
Wu, Changjun [1 ]
机构：
[1] Harbin Med Univ, Dept Ultrasound, Affiliated Hosp 1, Harbin 150001, Peoples R China
来源：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2016年 / 16卷 / 07期
关键词：
Ultrasound Imaging; Ultrasound Contrast Agents; Passive Targeting; Targeted Ligands; Atherosclerotic Plaques; DRUG-DELIVERY; NEOVASCULARIZATION; MICROBUBBLES; PERMEABILITY; VASCULATURE; VEGF;
D O I：
10.1166/jnn.2016.12033
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Targeting ultrasound contrast agents (UCAs) to specific areas of disease through specific ligands receptor binding has the potential to improve the ability to identify diseased tissue from healthy tissue. Herein, we report on the design and development of UCAs based on lipid nanoscale microbubbles (NBs) targeted to vulnerable vascular plaque for use in the diagnosis of atherosclerosis. Anti-VEGFR-2 ligands were conjuncted with NBs through biotin-avidin linkage process. The results indicated that the targeted NBs have a controlled size distribution in the desired nanoscale range. Fluorescence microscopy and flow cytometry analysis showed specific binding and high conjunction efficiency of targeted ligands to NBs. Then, the targeted NBs were applied to vivo ultrasound contrast imaging, and the results indicated the availability of ligands-conjuncted NBs in targeted ultrasound imaging and this availability has the potential to provide advantages for identifying atherosclerotic plaques.
引用
收藏
页码：7611 / 7616
页数：6
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