Eupatilin protects gastric epithelial cells from oxidative damage and down-regulates genes responsible for the cellular oxidative stress

被引：73

作者：

Choi, Eun-Ju ^{[1
]}

Oh, Hyun-Mee ^{[1
]}

Na, Bo-Ra ^{[1
,2
,3
]}

Ramesh, T. P. ^{[1
]}

Lee, Hyun-Ju ^{[1
]}

Choi, Chang-Soo ^{[4
]}

Choi, Suck-Chei ^{[4
]}

Oh, Tae-Young ^{[5
]}

Choi, Suck-Jun ^{[6
]}

Chae, Jeong-Ryong ^{[7
]}

Kim, Sang-Wook ^{[8
]}

Jun, Chang-Duk ^{[1
,2
,3
]}

机构：

[1] GIST, Dept Life Sci, Kwangju 500712, South Korea

[2] GIST, Cell Dynam Res Ctr, BioImaging Res Ctr, Kwangju 500712, South Korea

[3] GIST, Res Ctr Biomol Nanotechnol, Kwangju 500712, South Korea

[4] Wonkwang Univ, Sch Med, Digest Dis Res Inst, Iksan 570749, Chonbuk, South Korea

[5] Dong A Pharmaceut Co Ltd, Res Inst, Yongin 449905, South Korea

[6] Wonkwang Hlth Sci Coll, Dept Leisure Sports, Iksan 570749, Chonbuk, South Korea

[7] Kunsan Natl Univ, Dept Phys Educ, Chonbuk 573701, South Korea

[8] Chonbuk Natl Univ, Dept Internal Med, Jeonju 561712, South Korea

来源：

PHARMACEUTICAL RESEARCH | 2008年 / 25卷 / 06期

关键词：

anti-oxidant; eupatilin; microarray; reactive oxygen species; wound healing;

D O I：

10.1007/s11095-008-9531-5

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Purpose. The formulated ethanol extract (DA-9601) of Artemisia asiatica has pronounced anti-inflammatory activities and exhibits cytoprotective effects against gastrointestinal damage. Here we investigated whether eupatilin, a major component of DA-9601, has a property of antioxidant activity and protects gastric epithelial cells from H2O2-induced damage. Methods. The protective effect of eupatilin against H2O2-induced damages was studied in gastric epithelial AGS cells by measuring wound healing, cell proliferation, and cell viability. Global gene expression profiling was obtained by high-density microarray. Results. Hydrogen peroxide significantly delayed epithelial migration in wounded area. In contrast, eupatilin prevented the reduction of epithelial migration induced by H2O2. Eupatilin also ameliorated H2O2-induced actin disruption in AGS cells. Interestingly, treatment with eupatilin dramatically inhibited FeSO4-induced ROS production in a dose-dependent manner. In addition, eupatilin protected cells from FeSO4-induced F-actin disruption. With high-density microarray, we identified dozens of genes whose expressions were up-regulated in H2O2-treated cells. We found that eupatilin reduces the expression of such oxidative-responsible genes as HO-1, PLAUR and TNFRSF10A in H2O2-treated cells. Conclusion. These results suggest that eupatilin acts as a novel antioxidant and may play an important role in DA-9601-mediated effective repair of the gastric mucosa.

引用

页码：1355 / 1364

页数：10

共 39 条

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