Matrix-Assisted Laser Desorption-Ionization Imaging Mass Spectrometry for Direct Tissue Analysis

被引：0

作者：

Pallua, J. D.

Schaefer, G. ^{[2
]}

Bittner, L. K.

Pezzei, C.

Huck-Pezzei, V.

Schoenbichler, S. A.

Meding, S. ^{[3
]}

Rauser, S. ^{[3
]}

Walch, A. ^{[3
]}

Handler, M. ^{[4
]}

Netzer, M. ^{[4
]}

Osl, M. ^{[4
]}

Seger, M. ^{[4
]}

Pfeifer, B. ^{[4
]}

Baumgartner, C. ^{[4
]}

Lindner, H. ^{[5
]}

Kremser, L. ^{[5
]}

Sarg, B. ^{[5
]}

Klocker, H. ^{[2
]}

Bartsch, G. ^{[2
]}

Bonn, G. K.

Huck, C. W. ^{[1
]}

机构：

[1] Leopold Franzens Univ, Spect Grp, Inst Analyt Chem & Radiochem, A-6020 Innsbruck, Austria

[2] Med Univ Innsbruck, Dept Urol, Innsbruck, Austria

[3] Helmholtz Zentrum Munchen, Inst Pathol, Neuherberg, Germany

[4] Univ Hlth Sci Med Informat & Technol, Inst Elect Elect & Bioengn, In Hall, Austria

[5] Med Univ Innsbruck, Div Clin Biochem, Innsbruck, Austria

来源：

LC GC NORTH AMERICA | 2011年

关键词：

SAMPLE PREPARATION; MALDI-MS; CAPTURE MICRODISSECTION; SMALL MOLECULES; DRUG DISCOVERY; BREAST-CANCER; ION-TRAP; PROTEOMICS; PROTEINS; DESORPTION/IONIZATION;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Matrix-assisted laser desorption-ionization (MALDI) imaging mass spectrometry (IMS) is a powerful tool in histopathological characterization and represents a modern analytical technique, enabling two-dimensional detection of molecular components of biological samples. Using this method, it is possible to investigate the spatial distribution of proteins, lipids, carbohydrates, cholesterols, nucleic acids, phospholipids, and small molecules in biological systems by in-situ analysis of cell cultures and tissue sections. Recently, MALDI-IMS has become an essential tool for tissue analyses in life science applications, offering global analysis of tissue samples. An advantage of this imaging technique is the acquisition of local molecular expression profiles up to the microscopic level, while maintaining the topographic integrity of the tissue by avoiding time-consuming extraction, purification, or separation steps, respectively. With MALDI-IMS it is possible to determine the distribution of hundreds of unknown compounds in a single measurement, allowing rapid probing of the tissues' biochemistry. Moreover, MALDI-IMS results include qualitative and semiquantitative information, providing unique chemi-morphological information about the tissue status, which represents an important benefit for future analytical interpretation of pathological changes of a tissue. This article summarizes the most recent advances in sample preparation, instrumentation, and data-processing techniques for MALDI-IMS.

引用

页码：21 / 28

页数：8

共 61 条

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