Extracellular vesicles isolated from patients undergoing remote ischemic preconditioning decrease hypoxia-evoked apoptosis of cardiomyoblasts after isoflurane but not propofol exposure

被引：32

作者：

Abel, Frederik ^{[1
,2
]}

Murke, Florian ^{[2
]}

Gaida, Morten ^{[1
,2
]}

Garnier, Nicolas ^{[1
,2
]}

Ochsenfarth, Crista ^{[3
]}

Theiss, Carsten ^{[4
]}

Thielmann, Matthias ^{[2
,5
]}

Kleinbongard, Petra ^{[2
,6
]}

Giebel, Bernd ^{[2
]}

Peters, Juergen ^{[1
,2
]}

Frey, Ulrich H. ^{[1
,2
,3
]}

机构：

[1] Univ Duisburg Essen, Klin Anasthesiol & Intens Med, Essen, Germany

[2] Univ Klinikum Essen, Essen, Germany

[3] Univ Klinikum Ruhr Univ Bochum, Klin Anasthesiol Operat Intens Med Schmerz & Pall, Marien Hosp Herne, Bochum, Germany

[4] Ruhr Univ Bochum, Inst Anat, Abt Cytol, Bochum, Germany

[5] Univ Duisburg Essen, Klin Thorax & Kardiovaskulare Chirurg, Essen, Germany

[6] Univ Duisburg Essen, Inst Pathophysiol, Essen, Germany

来源：

PLOS ONE | 2020年 / 15卷 / 02期

关键词：

ENDOTHELIAL-CELLS; RAT-HEART; EXOSOMES; CARDIOPROTECTION; PROTECTION; DEPRIVATION; SURGERY;

D O I：

10.1371/journal.pone.0228948

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Remote ischemic preconditioning (RIPC) can evoke cardioprotection following ischemia/reperfusion and this may depend on the anesthetic used. We tested whether 1) extracellular vesicles (EVs) isolated from humans undergoing RIPC protect cardiomyoblasts against hypoxia-induced apoptosis and 2) this effect is altered by cardiomyoblast exposure to isoflurane or propofol. EVs were isolated before and 60 min after RIPC or Sham from ten patients undergoing coronary artery bypass graft surgery with isoflurane anesthesia and quantified by Nanoparticle Tracking Analysis. Following EV-treatment for 6 hours under exposure of isoflurane or propofol, rat H9c2 cardiomyoblasts were cultured for 18 hours in normoxic or hypoxic atmospheres. Apoptosis was detected by flow cytometry. Serum nanoparticle concentrations in patients had increased sixty minutes after RIPC compared to Sham (2.5x10(11)+/- 4.9x10(10) nanoparticles/ml; Sham: 1.2x10(11)+/- 2.0x10(10); p = 0.04). Hypoxia increased apoptosis of H9c2 cells (hypoxia: 8.4%+/- 0.6; normoxia: 2.5%+/- 0.1; p<0.0001). RIPC-EVs decreased H9c2 cell apoptosis compared to control (apoptotic ratio: 0.83; p = 0.0429) while Sham-EVs showed no protection (apoptotic ratio: 0.97). Prior isoflurane exposure in vitro even increased protection (RIPC-EVs/control, apoptotic ratio: 0.79; p = 0.0035; Sham-EVs/control, apoptotic ratio:1.04) while propofol (50 mu M) abrogated protection by RIPC-EVs (RIPC-EVs/control, Apoptotic ratio: 1.01; Sham-EVs/control, apoptotic ratio: 0.94; p = 0.602). Thus, EVs isolated from patients undergoing RIPC under isoflurane anesthesia protect H9c2 cardiomyoblasts against hypoxia-evoked apoptosis and this effect is abrogated by propofol. This supports a role of human RIPC-generated EVs in cardioprotection and underlines propofol as a possible confounder in RIPC-signaling mediated by EVs.

引用

页数：17

共 44 条

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