The thermal unfolding of native cytochrome c in the transition from solution to gas phase probed by native electron capture dissociation

被引：60
|
作者：
Breuker, K
McLafferty, FW
机构：
[1] Univ Innsbruck, Inst Organ Chem, A-6020 Innsbruck, Austria
[2] Univ Innsbruck, CMBI, A-6020 Innsbruck, Austria
[3] Cornell Univ, Baker Lab, Dept Chem & Chem Biol, Ithaca, NY 14853 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 31期
关键词：
hydration; mass spectrometry; native electron capture dissociation; noncovalent interactions; protein structures;
D O I：
10.1002/anie.200500668
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Unfolding upon dehydration:. What happens to the structure of a native protein that is suddenly exposed to vacuum? Native electron capture dissociation shows that dehydration turns the strongest intramolecular interactions in solution into the weakest in the gas phase. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：4911 / 4914
页数：4
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