A high resolution map of a cyanobacterial transcriptome

被引：87

作者：

Vijayan, Vikram ^{[1
,2
,3
]}

Jain, Isha H. ^{[2
,3
]}

O'Shea, Erin K. ^{[1
,2
,3
]}

机构：

[1] Harvard Univ, Grad Program Syst Biol, Cambridge, MA 02138 USA

[2] Harvard Univ, Howard Hughes Med Inst, Harvard Fac Arts & Sci, Dept Mol & Cellular Biol,Ctr Syst Biol, Cambridge, MA 02138 USA

[3] Harvard Univ, Howard Hughes Med Inst, Harvard Fac Arts & Sci, Dept Chem & Chem Biol,Ctr Syst Biol, Cambridge, MA 02138 USA

来源：

GENOME BIOLOGY | 2011年 / 12卷 / 05期

基金：

美国国家科学基金会;

关键词：

CIRCADIAN GENE-EXPRESSION; POLYMERASE BINDING-SITE; ESCHERICHIA-COLI; RNA-POLYMERASE; SYNECHOCOCCUS-ELONGATUS; NUCLEOTIDE-SEQUENCE; START SITES; IN-VIVO; DNA; GENOME;

D O I：

10.1186/gb-2011-12-5-r47

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Previous molecular and mechanistic studies have identified several principles of prokaryotic transcription, but less is known about the global transcriptional architecture of bacterial genomes. Here we perform a comprehensive study of a cyanobacterial transcriptome, that of Synechococcus elongatus PCC 7942, generated by combining three high-resolution data sets: RNA sequencing, tiling expression microarrays, and RNA polymerase chromatin immunoprecipitation sequencing. Results: We report absolute transcript levels, operon identification, and high-resolution mapping of 5' and 3' ends of transcripts. We identify several interesting features at promoters, within transcripts and in terminators relating to transcription initiation, elongation, and termination. Furthermore, we identify many putative non-coding transcripts. Conclusions: We provide a global analysis of a cyanobacterial transcriptome. Our results uncover insights that reinforce and extend the current views of bacterial transcription.

引用

页数：17

共 63 条

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