Lycium barbarum polysaccharide improves the development of mouse oocytes vitrified at the germinal vesicle stage

被引：17
作者：
Yang, Lei [1 ,3 ]
Lei, Lanjie [2 ]
Zhao, Qihan [1 ]
Gao, Zhen [4 ]
Xu, Xiaoyuan [1 ,3 ]
机构：
[1] Jiujiang Univ, Coll Basic Med Sci, Jiujiang 332000, Jiangxi, Peoples R China
[2] Jiujiang Univ, Affiliated Hosp, Jiujiang 332000, Jiangxi, Peoples R China
[3] Jiujiang Univ, Key Lab Syst Biomed Jiangxi Prov, Jiujiang 332000, Jiangxi, Peoples R China
[4] Northwest A&F Univ, Minist Agr, Key Lab Anim Biotechnol, Yangling 712100, Shaanxi, Peoples R China
来源：
CRYOBIOLOGY | 2018年 / 85卷
关键词：
Lycium barbarum polysaccharide; Endoplasmic reticulum stress; AKT; MAPK3/1; Vitrification; VITRIFICATION; MATURATION; ACTIVATION; EXPRESSION;
D O I：
10.1016/j.cryobiol.2018.10.265
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The cryopreservation of cumulus oocyte complexes (COCs) is an important process in reproductive medicine. However, this process slows both embryo and offspring development. In this study, the effects of maturation medium supplementation with lycium barbarum polysaccharide (LBP) on the developmental competence of vitrified-warmed mouse COCs and the possible mechanisms facilitating this process were investigated. The addition of LBP to the maturation medium improved the maturation rate and enhanced the developmental competence of vitrified-warmed mouse COCs. This may reduce endoplasmic reticulum stress (ERS), preventing cell death and activating both PI3K/AKT and MAPK3/1. In conclusion, cryopreservation lowers the maturation rate and developmental competence of COCs, which can be ameliorated by the application of LBP after warming during maturation.
引用
收藏
页码：7 / 11
页数：5
相关论文
共 40 条
  • [1] Autophagic activation in vitrified-warmed mouse oocytes
    Bang, Soyoung
    Shin, Hyejin
    Song, Haengseok
    Suh, Chang Suk
    Lim, Hyunjung Jade
    [J]. REPRODUCTION, 2014, 148 (01) : 11 - 19
  • [2] Use of anti-aging herbal medicine, Lycium barbarum, against aging-associated diseases.: What do we know so far?
    Chang, Raymond Chuen-Chung
    So, Kwok-Fai
    [J]. CELLULAR AND MOLECULAR NEUROBIOLOGY, 2008, 28 (05) : 643 - 652
  • [3] Effect of Lycium Barbarum (Wolfberry) Polysaccharides on Preserving Retinal Function after Partial Optic Nerve Transection
    Chu, Patrick H. W.
    Li, Hong-Ying
    Chin, Man-Pan
    So, Kwok-fai
    Chan, Henry H. L.
    [J]. PLOS ONE, 2013, 8 (12):
  • [4] Akt and Autophagy Cooperate to Promote Survival of Drug-Resistant Glioma
    Fan, Qi-Wen
    Cheng, Christine
    Hackett, Chris
    Feldman, Morri
    Houseman, Benjamin T.
    Nicolaides, Theodore
    Haas-Kogan, Daphne
    James, C. David
    Oakes, Scott A.
    Debnath, Jayanta
    Shokat, Kevan M.
    Weiss, William A.
    [J]. SCIENCE SIGNALING, 2010, 3 (147)
  • [5] Lycium barbarum polysaccharide attenuates the cytotoxicity of mutant huntingtin and increases the activity of AKT
    Fang, Fang
    Peng, Ting
    Yang, Shiming
    Wang, Weixi
    Zhang, Yinong
    Li, He
    [J]. INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, 2016, 52 : 66 - 74
  • [6] Effect of in vitro fertilization on gene expression and development of mouse preimplantation embryos
    Giritharan, Gnanaratnam
    Talbi, Said
    Donjacour, Annemarie
    Di Sebastiano, Francesca
    Dobson, Anthony T.
    Rinaudo, Paolo F.
    [J]. REPRODUCTION, 2007, 134 (01) : 63 - 72
  • [7] Slow cooling of human oocytes: ultrastructural injuries and apoptotic status
    Gualtieri, Roberto
    Iaccarino, Mirella
    Mollo, Valentina
    Prisco, Marina
    Iaccarino, Stefania
    Talevi, Riccardo
    [J]. FERTILITY AND STERILITY, 2009, 91 (04) : 1023 - 1034
  • [8] Vitrification of mouse germinal vesicle oocytes: effect of treatment temperature and egg yolk on chromatin and spindle normality and cumulus integrity
    Isachenko, EF
    Nayudu, PL
    [J]. HUMAN REPRODUCTION, 1999, 14 (02) : 400 - 408
  • [9] Aseptic vitrification of human germinal vesicle oocytes using dimethyl sulfoxide as a cryoprotectant
    Isachenko, V
    Montag, M
    Isachenko, E
    Dessole, S
    Nawroth, F
    van der Ven, H
    [J]. FERTILITY AND STERILITY, 2006, 85 (03) : 741 - 747
  • [10] Lycium barbarum polysaccharide attenuates high-fat diet-induced hepatic steatosis by up-regulating SIRT1 expression and deacetylase activity
    Jia, Li
    Li, Wang
    Li, Jianning
    Li, Yan
    Song, Hui
    Luan, Yansong
    Qi, Hui
    Ma, Lirong
    Lu, Xiaohong
    Yang, Yi
    [J]. SCIENTIFIC REPORTS, 2016, 6
1234