Effectiveness of Antimicrobial Photodynamic Therapy on Staphylococcus aureus using Phenothiazinium Dye with Red Laser.

被引：3
|
作者：
Monteiro, Juliana S. C. [1 ,2 ,3 ]
de Oliveira, Susana C. P. S. [1 ,2 ]
Pires-Santos, Gustavo M. [4 ]
Pires Sampaio, Fernando Jose [1 ,2 ]
Aparecida Zanin, Fatima Antonia [2 ,5 ]
Pinheiro, Antonio L. B. [1 ,2 ,5 ]
机构：
[1] Univ Fed Bahia, Sch Dent, Ctr Biophoton, BR-40110150 Salvador, BA, Brazil
[2] Natl Inst Opt & Photon, BR-13560970 Sao Carlos, SP, Brazil
[3] Estate Univ Feira de Santana, Dept Biol, BR-44036900 Feira De Santana, BA, Brazil
[4] Fiocruz MS, INCT INPeTAM, Fundacao Oswaldo Cruz, Ctr Pesquisas Goncalo Muniz,Lab Parasite Biol, Salvador, BA, Brazil
[5] Brugnera & Zanin Inst, BR-01434000 Sao Paulo, SP, Brazil
来源：
MECHANISMS FOR LOW-LIGHT THERAPY X | 2015年 / 9309卷
关键词：
Photodynamic antimicrobial chemotherapy; Staphylococcus aureus; lasers; METHICILLIN-RESISTANT; IRRADIATION; STRAINS;
D O I：
10.1117/12.2079170
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
The aim of this study was to evaluate in vitro the bactericidal effect of Antimicrobial Photodynamic Therapy - AmPDT using a phenothiazinium compound (toluidine blue O and methylene blue, 12.5 mu g/mL) on Staphylococcus aureus (ATCC 23529) irradiated or not with the red laser (lambda 660 nm, 12J/cm(2)). All tests were performed in triplicate and samples distributed into the following groups: Negative control, Laser, Photosensitizer, and AmPDT. Bactericidal effect of the Antimicrobial Photodynamic Therapy was assessed by counting of colony-forming units and analyzed statistically (ANOVA, Tukey test, p<0.05). The results showed, comparing the Laser group with Negative control, a statistically significant increase of counting on the Laser group (p = 0.003). The use of the photosensitizer alone reduced the mean number of CFU (64.8%) and its association with the Laser light resulted in 84.2% of inhibition. The results are indicative that the use of Antimicrobial Photodynamic Therapy presented in vitro bactericidal effect on Staphylococcus aureus.
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页数：6
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