Anti-oxidant and anti-cancer activities of Angelica dahurica extract via induction of apoptosis in colon cancer cells

被引：59
作者：
Zheng, Yi Mei [1 ]
Shen, James Zheng [2 ]
Wang, Yan [2 ]
Lu, Amy Xiaoxu [2 ]
Ho, Wing Shing [2 ]
机构：
[1] Minnan Normal Univ, Coll Biol Sci & Technol, Zhangzhou 363000, Peoples R China
[2] Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R China
来源：
PHYTOMEDICINE | 2016年 / 23卷 / 11期
关键词：
Angelica dahurica Radix; Colon cancer; Apoptosis; Cell cycle arrest; ROS scavenger; IMPERATORIN; RADIX; CHROMATOGRAPHY; MECHANISM;
D O I：
10.1016/j.phymed.2015.11.008
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Introduction: Angelica dahurica Radix is the common herbal medicine with anti-cancer activities. However, details of its anti-cancer activities are lacking. Materials and methods: We investigated the anti-cancer effects of Angelica dahurica extract in HT-29 colon cancer cell line. Cell viability, apoptotic and necrotic activities and the mechanism of actions of the active fraction were measured. Results and discussion: The organic extract of Angelica dahurica Radi decreased significantly the gene expression of p53, Bcl, Bax and induced apoptosis via caspase cascade and cell cycle arrest. The ethanol-ethyl acetate fraction showed anti-cancer activities in HT-29 cancer cells. A HPLC-DAD analysis of the fraction indicated the presence of Imperatorin and isoimperatorin, which are the major coumarins in the active fraction that contribute to the anti-cancer activities. Conclusions: This study has evaluated the ant-cancer activity of the organic extract of Angelica dahurica Radix against colon cancer cells and provided a basis of further development of the herbal extract for treatment of colon cancer. (C) 2015 Elsevier GmbH. All rights reserved.
引用
收藏
页码：1267 / 1274
页数：8
相关论文
共 15 条
  • [1] MODFIT - A PHARMACOKINETICS COMPUTER-PROGRAM
    ALLEN, GD
    [J]. BIOPHARMACEUTICS & DRUG DISPOSITION, 1990, 11 (06) : 477 - 498
  • [2] Antioxidant effect of imperatorin from Angelica dahurica in hypertension via inhibiting NADPH oxidase activation and MAPK pathway
    Cao, Yanjun
    Zhang, Yanmin
    Wang, Nan
    He, Langchong
    [J]. JOURNAL OF THE AMERICAN SOCIETY OF HYPERTENSION, 2014, 8 (08) : 527 - 536
  • [3] Chen Y., 2012, ADV CHEM ENG, VII, P550
  • [4] Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells
    Jeong, Su-Hyeon
    Kim, Bo-Bae
    Lee, Ji-Eun
    Ko, Youngkyung
    Park, Jun-Beom
    [J]. MOLECULAR MEDICINE REPORTS, 2015, 12 (01) : 1556 - 1560
  • [5] Development of an analytical method coupling cell membrane chromatography with gas chromatography-mass spectrometry via microextraction by packed sorbent and its application in the screening of volatile active compounds in natural products
    Li, Miao
    Wang, Sicen
    He, Langchong
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2015, 974 : 9 - 16
  • [6] Quantitative Evaluation of the Mechanism Underlying the Biotransportation of the Active Ingredients in Puerariaelobatae Radix and Chuanxiongrhizoma
    Liang, Xin-Li
    Zhang, Jing
    Liao, Zheng-Gen
    Zhao, Guo-Wei
    Luo, Yun
    Li, Zhe
    Satterlee, Andrew
    [J]. PHYTOTHERAPY RESEARCH, 2015, 29 (09) : 1396 - 1403
  • [7] Anticancer Effects of Imperatorin Isolated from Angelica dahurica: Induction of Apoptosis in HepG2 Cells through both Death-Receptor- and Mitochondria-Mediated Pathways
    Luo, Ke-wang
    Sun, Jian-guo
    Chan, Judy Yuet-Wa
    Yang, Lu
    Wu, Shi-hua
    Fung, Kwok-Pui
    Liu, Fei-yan
    [J]. CHEMOTHERAPY, 2011, 57 (06) : 449 - 459
  • [8] Imperatorin Is Responsible for the Vasodilatation Activity of Angelica Dahurica var. Formosana Regulated by Nitric Oxide in an Endothelium-Dependent Manner
    Nie Hong
    Meng Lan-zhen
    Zhou Jiu-yao
    Fan Xiao-feng
    Luo-Yong
    Zhang Guang-wen
    [J]. CHINESE JOURNAL OF INTEGRATIVE MEDICINE, 2009, 15 (06) : 442 - 447
  • [9] Role of p53 and reactive oxygen species in apoptotic response to copper and zinc in epithelial breast cancer cells
    Ostrakhovitch, EA
    Cherian, MG
    [J]. APOPTOSIS, 2005, 10 (01) : 111 - 121
  • [10] Simultaneous determination of five coumarins in Angelicae dahuricae Radix by HPLC/UV and LC-ESI-MS/MS
    Park, Ah Yeon
    Park, So-Young
    Lee, Jaehyun
    Jung, Mihye
    Kim, Jinwoong
    Kang, Sam Sik
    Youm, Jeong-Rok
    Han, Sang Beom
    [J]. BIOMEDICAL CHROMATOGRAPHY, 2009, 23 (10) : 1034 - 1043
12