A Pan-plant Protein Complex Map Reveals Deep Conservation and Novel Assemblies

被引:135
作者
McWhite, Claire D. [1 ]
Papoulas, Ophelia [1 ]
Drew, Kevin [1 ]
Cox, Rachael M. [1 ]
June, Viviana [1 ]
Dong, Oliver Xiaoou [2 ,3 ,4 ]
Kwon, Taejoon [5 ]
Wan, Cuihong [1 ,6 ]
Salmi, Mari L. [1 ]
Roux, Stanley J. [1 ]
Browning, Karen S. [1 ]
Chen, Z. Jeffrey [1 ]
Ronald, Pamela C. [2 ,3 ,4 ]
Marcotte, Edward M. [1 ]
机构
[1] Univ Texas Austin, Ctr Syst & Synthet Biol, Dept Mol Biosci, Austin, TX 78712 USA
[2] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
[3] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA
[4] Joint Bioenergy Inst, Emeryville, CA 94608 USA
[5] UNIST, Sch Life Sci, Dept Biomed Engn, 50 UNIST Gil, Ulsan 44919, South Korea
[6] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luoyu Rd, Wuhan 430079, Peoples R China
关键词
SCALE GENE NETWORK; HUMAN INTERACTOME; ANION CHANNEL; GENOME; MULTIPLE; EVOLUTION; SIZE; TRANSCRIPTION; PURIFICATION; ASSOCIATION;
D O I
10.1016/j.cell.2020.02.049
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plants are foundational for global ecological and economic systems, but most plant proteins remain uncharacterized. Protein interaction networks often suggest protein functions and open new avenues to characterize genes and proteins. We therefore systematically determined protein complexes from 13 plant species of scientific and agricultural importance, greatly expanding the known repertoire of stable protein complexes in plants. By using co-fractionation mass spectrometry, we recovered known complexes, confirmed complexes predicted to occur in plants, and identified previously unknown interactions conserved over 1.1 billion years of green plant evolution. Several novel complexes are involved in vernalization and pathogen defense, traits critical for agriculture. We also observed plant analogs of animal complexes with distinct molecular assemblies, including a megadalton-scale tRNA multi-synthetase complex. The resulting map offers a cross-species view of conserved, stable protein assemblies shared across plant cells and provides a mechanistic, biochemical framework for interpreting plant genetics and mutant phenotypes.
引用
收藏
页码:460 / +
页数:29
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