Development, characterization, and inheritance of 113 novel EST-SSR markers in the Pacific oyster (Crassostrea gigas)

被引：16

作者：

Li, Qi ^{[1
]}

Wang, Qingzhi ^{[1
]}

Qi, Mingjun ^{[1
]}

Ge, Jianlong ^{[1
]}

Cong, Rihao ^{[1
]}

机构：

[1] Ocean Univ China, Key Lab Mariculture, Minist Educ, Qingdao 266003, Peoples R China

来源：

GENES & GENOMICS | 2011年 / 33卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Pacific oyster; Crassotrea gigas; EST-SSR markers; MICROSATELLITE DNA MARKERS; EXPRESSED SEQUENCE TAGS; NULL ALLELES; LOCI; IDENTIFICATION;

D O I：

10.1007/s13258-011-0021-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A total of 113 novel EST-derived simple sequence repeat (EST-SSR) markers were developed in the Pacific oyster (Crassotrea gigas). Polymorphisms of these markers were evaluated in a wild population of 30 individuals. The number of alleles per locus ranged from 2 to 27 with an average of 6.3, and the observed and expected heterozygosities varied from 0 to 0.9667 and from 0.0333 to 0.9701, respectively. Mendelian segregations were tested for 24 of the markers that were polymorphic in one family produced by single-pair mating. Null alleles were discovered at four loci. Nine tests of segregation ratios revealed significant departures from expected Mendelian ratios. As a useful addition to the collection of the microsatellites that are now available for C. gigas, these EST-SSR markers will help the advance in investigation of QTL mapping and genetic diversity in this species.

引用

页码：313 / 316

页数：4

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