Lacibacter luteus sp. nov., isolated from a freshwater river

An isolate, designated TTM-7(T), recovered from a freshwater river in Taiwan, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that strain TTM-7(T) is affiliated with species in the genus Lacibacter. Strain TTM-7(T) was most closely related to Lacibacter cauensis NJ-8(T) with a 98.2% 16S rRNA gene sequence similarity, followed by Lacibacter daechungensis H32-4(T) (97.4%) and Lacibacter nakdongensis SS2-56(T) (96.3%). Cells were Gram-stain-negative, aerobic, motile by gliding, rod-shaped and formed orange colonies. Optimal growth occurred at 25 degrees C, pH 7 and 0% NaCl. The major fatty acids of strain TTM-7(T) were iso-C-15:0, summed feature 3 (comprising C-16:1 omega 7c/C-16:1 omega 6c and/or iso-C-15:0 2-OH) and iso-C-15:1 G. The predominant hydroxy fatty acid was iso-C-17:0 3-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, two unidentified aminoglycolipids, five unidentified aminophospholipids and one unidentified lipid. The major isoprenoid quinone was MK-7. Genomic DNA G+C content of strain TTM-7(T) was 40.6 mol%. Strain TTM-7(T) showed 83.6% average nucleotide identity and 16.0% digital DNA-DNA hybridization identity with Lacibacter cauensis NJ-8(T). On the basis of phenotypic and genotypic properties and phylogenetic inference, strain TTM-7(T) should be classified in a novel species of the genus Lacibacter, for which the name Lacibacter luteus sp. nov. is proposed. The type strain is TTM-7(T) (=BCRC 81159(T)=LMG 30926(T)=KCTC 62870(T)).