Identification of Saliva Using MicroRNA Biomarkers for Forensic Purpose

被引：38

作者：

Wang, Zheng ^{[1
]}

Zhang, Ji ^{[1
]}

Wei, Wei ^{[1
]}

Zhou, Di ^{[1
]}

Luo, Haibo ^{[1
]}

Chen, Xiaogang ^{[1
,2
]}

Hou, Yiping ^{[1
]}

机构：

[1] West China Univ Med Sci, Dept Forens Genet, West China Sch Preclin & Forens Med, Sichuan Univ, Chengdu 610041, Sichuan, Peoples R China

[2] West China Univ Med Sci, Dept Forens Pathol, West China Sch Preclin & Forens Med, Sichuan Univ, Chengdu 610041, Sichuan, Peoples R China

来源：

JOURNAL OF FORENSIC SCIENCES | 2015年 / 60卷 / 03期

关键词：

forensic science; forensic genetics; body fluid identification; saliva; microRNA; TaqMan; QUANTITATIVE PCR; RNA; EXPRESSION; MARKERS; BLOOD; DNA;

D O I：

10.1111/1556-4029.12730

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

In the forensic science community, microRNA (miRNA) profiling has started to be explored as an alternative tool for body fluid identification. Several origins of body fluid can be distinguished by measuring differential expression patterns of particular miRNAs. However, most of reported saliva miRNAs are nonoverlapping and debatable. The aim of this study was to develop a strategy of identifying saliva using miRNA biomarkers for forensic purpose. Eight miRNA candidates were selected to examine expression abundance in forensically relevant body fluids using hydrolysis probes quantitative real-time PCR (TaqMan qPCR). Results revealed that none of them was truly saliva specific, and only miR-200c-3p, miR-203a, and miR-205-5p were higher or more moderate expression in saliva. A stepwise strategy that combines each of three miRNAs with different body fluid-specific miRNAs was developed, and three miRNA combinations could effectively differentiate saliva from other body fluids.

引用

页码：702 / 706

页数：5

共 17 条

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