Pullulan production by an osmotolerant Aureobasidium pullulans RBF-4A3 isolated from flowers of Caesulia axillaris

被引：58

作者：

Choudhury, Anirban Roy ^{[2
]}

Saluja, Puja ^{[1
]}

Prasad, G. S. ^{[1
]}

机构：

[1] CSIR, Microbial Type Culture Collect & Gene Bank MTCC, Inst Microbial Technol IMTECH, Sector 39A, Chandigarh 160036, India

[2] CSIR, BERPDC, Inst Microbial Technol IMTECH, Chandigarh 160036, India

来源：

CARBOHYDRATE POLYMERS | 2011年 / 83卷 / 04期

关键词：

Aureobasidium pullulans; Fermentation; Exopolysaccharide; Pullulan; Osmotolerant; MOLECULAR-WEIGHT PULLULAN; SP NOV; YEASTS; OPTIMIZATION; STRAIN; BATCH; PHYLLOPLANE;

D O I：

10.1016/j.carbpol.2010.10.003

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Phenotypic and molecular characterization of five yeast-like fungal isolates from flowers of wild plants showed that they are related to Aureobasidium pullulans. Compared to other isolates, an osmotolerant and non-pigmented isolate A. pullulans RBF-4A3 produced 26.35 gl(-1) of melanin-free exopolysaccharide (EPS) in 96h at 30 degrees C in 5% glucose containing medium. At higher concentrations of glucose (7.5-25% (w/v)), the EPS produced by this organism increased from 34.68 to 66.79 gl(-1) up to 15% (w/v) glucose, with a productivity of 16.69 gl(-1) per day. Beyond 15% (w/v) glucose concentration, the EPS production decreased gradually to 43.29 gl(-1) at 25% (w/v) glucose. Fourier-transform infrared (FTIR) spectroscopy confirmed that chemical structures of the exopolysaccharicle produced by A. pullulans RBF-4A3 and standard pullulan were identical. This is the first report of pullulan production at 15% (w/v) concentration of glucose by an osmotolerant strain of A. pullulans. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1547 / 1552

页数：6