Pullulan production by an osmotolerant Aureobasidium pullulans RBF-4A3 isolated from flowers of Caesulia axillaris

被引：58
作者：
Choudhury, Anirban Roy [2 ]
Saluja, Puja [1 ]
Prasad, G. S. [1 ]
机构：
[1] CSIR, Microbial Type Culture Collect & Gene Bank MTCC, Inst Microbial Technol IMTECH, Sector 39A, Chandigarh 160036, India
[2] CSIR, BERPDC, Inst Microbial Technol IMTECH, Chandigarh 160036, India
来源：
CARBOHYDRATE POLYMERS | 2011年 / 83卷 / 04期
关键词：
Aureobasidium pullulans; Fermentation; Exopolysaccharide; Pullulan; Osmotolerant; MOLECULAR-WEIGHT PULLULAN; SP NOV; YEASTS; OPTIMIZATION; STRAIN; BATCH; PHYLLOPLANE;
D O I：
10.1016/j.carbpol.2010.10.003
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Phenotypic and molecular characterization of five yeast-like fungal isolates from flowers of wild plants showed that they are related to Aureobasidium pullulans. Compared to other isolates, an osmotolerant and non-pigmented isolate A. pullulans RBF-4A3 produced 26.35 gl(-1) of melanin-free exopolysaccharide (EPS) in 96h at 30 degrees C in 5% glucose containing medium. At higher concentrations of glucose (7.5-25% (w/v)), the EPS produced by this organism increased from 34.68 to 66.79 gl(-1) up to 15% (w/v) glucose, with a productivity of 16.69 gl(-1) per day. Beyond 15% (w/v) glucose concentration, the EPS production decreased gradually to 43.29 gl(-1) at 25% (w/v) glucose. Fourier-transform infrared (FTIR) spectroscopy confirmed that chemical structures of the exopolysaccharicle produced by A. pullulans RBF-4A3 and standard pullulan were identical. This is the first report of pullulan production at 15% (w/v) concentration of glucose by an osmotolerant strain of A. pullulans. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1547 / 1552
页数：6
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