High-throughput approaches to profile RNA-protein interactions

被引：31

作者：

Nechay, Misha ^{[1
]}

Kleiner, Ralph E. ^{[1
]}

机构：

[1] Princeton Univ, Dept Chem, Princeton, NJ 08544 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2020年 / 54卷

关键词：

Ribonomics; Transcriptomics; Proteomics; CLIP; TRANSCRIPTOME-WIDE DISCOVERY; HETEROGENEOUS NUCLEAR-RNA; BINDING SITES; BOUND PROTEOME; CROSS-LINKING; IDENTIFICATION; RESOLUTION; RIBONUCLEOPROTEIN; PARTICLES; NETWORKS;

D O I：

10.1016/j.cbpa.2019.11.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA-protein interactions play a critical role in post-transcriptional gene regulation. Characterizing these interactions in their native context has been challenging; however, advances in RNA sequencing and mass spectrometrybased proteomics combined with innovative chemical biological tools have heralded the development of robust strategies for performing biochemistry on a cellular scale. Herein, we review recent advances in the development and application of proteomic and transcriptomic approaches to profile cellular RNA-protein interactions, focusing on sequencing-based strategies and proteomic analysis of RNA-binding proteins, as well as approaches to address the role of RNA modifications in protein-RNA binding events.

引用

页码：37 / 44

页数：8

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