Biological pH sensing based on surface enhanced Raman scattering through a 2-aminothiophenol-silver probe

被引：75

作者：

Wang, Zhuyuan ^{[1
]}

Bonoiu, Adela ^{[1
]}

Samoc, Marek ^{[1
]}

Cui, Yiping ^{[2
]}

Prasad, Paras N. ^{[1
]}

机构：

[1] SUNY Buffalo, Inst Lasers Photon & Biophoton, Buffalo, NY 14260 USA

[2] SE Univ, Adv Photon Ctr, Nanjing 210096, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2008年 / 23卷 / 06期

关键词：

pH sensor; SERS probe; cellular imaging; surface enhanced Raman scattering;

D O I：

10.1016/j.bios.2007.09.017

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We report pH sensing for biological applications based on surface enhanced Raman scattering (SERS) from silver nanoparticles functionalized with 2-aminothiophenol (2-aminobenzenethiol, 2-ABT). pH-dependent SERS spectra of the attached 2-ABT molecules enable one to sense the pH over the range of 3.0-8.0. We have performed the first demonstration of SERS detection in living cells kept in different pH buffer solutions and show that the pH sensitivity is retained in that case. Thus, the nanoparticles can be used as probes delivering spatially localized chemical information from biological environments and provide a new way to monitor chemical changes at cellular level. (C) 2007 Published by Elsevier B.V.

引用

页码：886 / 891

页数：6

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