Mapping the Fine-Scale Organization and Plasticity of the Brain Vasculature

被引：238

作者：

Kirst, Christoph ^{[1
,2
,3
,4
,5
]}

Skriabine, Sophie ^{[1
]}

Vieites-Prado, Alba ^{[1
]}

Topilko, Thomas ^{[1
]}

Bertin, Paul ^{[1
]}

Gerschenfeld, Gaspard ^{[6
]}

Verny, Florine ^{[1
]}

Topilko, Piotr ^{[6
]}

Michalski, Nicolas ^{[7
]}

Tessier-Lavigne, Marc ^{[8
]}

Renier, Nicolas ^{[1
]}

机构：

[1] Sorbonne Univ, CNRS, UMR7225,Lab Plast Struct, INSERM,U1127,ICM Inst Cerveau & Moelle Epiniere, F-75013 Paris, France

[2] Rockefeller Univ, Ctr Phys & Biol, New York, NY 10065 USA

[3] Rockefeller Univ, Kavli Neural Syst Inst, New York, NY 10065 USA

[4] Univ Calif San Francisco, Kavli Inst Fundamental Neurosci, Sandler Neurosci Bldg,Suite 514G, San Francisco, CA 94158 USA

[5] Univ Calif San Francisco, Anat Dept, Sandler Neurosci Bldg,Suite 514G, San Francisco, CA 94158 USA

[6] INSERM, U955, Inst Mondor Rech Biomed, Team 9, Creteil, France

[7] Inst Pasteur, INSERM, UMRS 1120, Unite Genet & Physiol Audit, F-75015 Paris, France

[8] Stanford Univ, Dept Biol, Stanford, CA 94305 USA

来源：

CELL | 2020年 / 180卷 / 04期

基金：

欧盟地平线“2020”; 欧洲研究理事会;

关键词：

FOCAL CEREBRAL-ISCHEMIA; BLOOD-FLOW; ALZHEIMERS-DISEASE; NEURAL ACTIVITY; CORTEX; RAT; QUANTIFICATION; ANGIOGENESIS; PODOCALYXIN; OTOFERLIN;

D O I：

10.1016/j.cell.2020.01.028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cerebral vasculature is a dense network of arteries, capillaries, and veins. Quantifying variations of the vascular organization across individuals, brain regions, or disease models is challenging. We used immunolabeling and tissue clearing to image the vascular network of adult mouse brains and developed a pipeline to segment terabyte-sized multi-channel images from light sheet microscopy, enabling the construction, analysis, and visualization of vascular graphs composed of over 100 million vessel segments. We generated datasets from over 20 mouse brains, with labeled arteries, veins, and capillaries according to their anatomical regions. We characterized the organization of the vascular network across brain regions, highlighting local adaptations and functional correlates. We propose a classification of cortical regions based on the vascular topology. Finally, we analysed brain-wide rearrangements of the vasculature in animal models of congenital deafness and ischemic stroke, revealing that vascular plasticity and remodeling adopt diverging rules in different models.

引用

页码：780 / +

页数：41

共 77 条

[1] The pial vasculature of the mouse develops according to a sensory-independent program [J].