DNA barcoding of clinically relevant Cunninghamella species

被引：23
|
作者：
Yu, Jin [1 ,2 ]
Walther, G. [3 ,4 ]
Van Diepeningen, A. D. [2 ]
Van den Ende, A. H. G. Gerrits [2 ]
Li, Ruo-Yu [1 ]
Moussa, T. A. A. [5 ,6 ]
Almaghrabi, O. A. [5 ]
De Hoog, G. S. [1 ,2 ,5 ,7 ,8 ,9 ,10 ]
机构：
[1] Peking Univ, Hlth Sci Ctr, Dept Dermatol & Venereol, Hosp 1,Res Ctr forMed Mycol, Beijing 100871, Peoples R China
[2] CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands
[3] Univ Jena, Inst Microbiol, Dept Microbiol & Mol Biol, Jena, Germany
[4] Jena Microbial Resource Collect, Leibniz Inst Nat Prod Res & Infect Biol, Hans Knoll Inst, Jena, Germany
[5] King Abdulaziz Univ, Fac Sci North Jeddah, Biol Sci Dept, Jeddah 21413, Saudi Arabia
[6] Cairo Univ, Fac Sci, Dept Bot & Microbiol, Giza, Egypt
[7] Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands
[8] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou 510275, Guangdong, Peoples R China
[9] Second Mil Med Univ, Shanghai Inst Med Mycol, Changzheng Hosp, Shanghai, Peoples R China
[10] Univ Fed Parana, Basic Pathol Dept, Curitiba, Parana, Brazil
来源：
MEDICAL MYCOLOGY | 2015年 / 53卷 / 02期
关键词：
Cunninghamella; ITS; tef-1; alpha; mucormycosis; IMMUNOCOMPETENT PATIENT; MUCORALES; RHIZOPUS; RDNA;
D O I：
10.1093/mmy/myu079
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Mucormycosis caused, in part, by representatives of the genus Cunninghamella is a severe infection with high mortality in patients with impaired immunity. Several species have been described in the literature as etiologic agents. A DNA barcoding study using ITS rDNA and tef-1 alpha provided concordance of molecular data with conventional characters. The currently accepted Cunninghamella species were well supported in phylogenetic trees of both markers except for C. septata with ITS that clustered in the C. echinulata clade. Sequence variability was distinctly higher for the ITS than for tef-1a. Intraspecific ITS variability of some of the species exceeded that between some closely related species, but the marker remained applicable for species identification. The most variable species for both markers was C. echinulata. Cunninghamella bertholletiae is the main pathogenic species; infections by C. blakesleeana, C. echinulata, and C. elegans are highly exceptional.
引用
收藏
页码：99 / 106
页数：8
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