Methods for genetic transformation of filamentous fungi

被引：129
|
作者：
Li, Dandan [1 ,3 ]
Tang, Yu [4 ]
Lin, Jun [1 ,2 ,3 ]
Cai, Weiwen [1 ,3 ]
机构：
[1] Fuzhou Univ, Inst Apply Genom, 2 Xueyuan Rd, Fuzhou 350108, Fujian, Peoples R China
[2] Fujian Med Univ, Sch Basic Med Sci, 1 Xuefubei Rd, Fuzhou 350122, Fujian, Peoples R China
[3] Fuzhou Univ, Coll Biol Sci & Engn, 2 Xueyuan Rd, Fuzhou 350108, Fujian, Peoples R China
[4] Triplex Int Biosci China Co LTD, Xiamen 361100, Peoples R China
来源：
MICROBIAL CELL FACTORIES | 2017年 / 16卷
基金：
中国国家自然科学基金;
关键词：
Filamentous fungi; Protoplast-mediated transformation; Agrobacterium-mediated transformation; Electroporation; Biolistic method; Shock-wave-mediated transformation; AGROBACTERIUM-MEDIATED TRANSFORMATION; HIGH-EFFICIENCY TRANSFORMATION; ASPERGILLUS-NIDULANS; TRICHODERMA-REESEI; SHOCK-WAVES; ELECTROPORATION; TUMEFACIENS; PLASMID; DNA; SYSTEM;
D O I：
10.1186/s12934-017-0785-7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a precondition that enables scientists to target and modify genes efficiently and may reveal the function of target genes. The method to deliver foreign nucleic acid into cells is the sticking point for fungal genome modification. Up to date, there are some general methods of genetic transformation for fungi, including protoplast-mediated transformation, Agrobacterium-mediated transformation, electroporation, biolistic method and shock-wave-mediated transformation. This article reviews basic protocols and principles of these transformation methods, as well as their advantages and disadvantages.
引用
收藏
页数：13
相关论文
共 50 条
  • [21] Methods for genetic transformation in Dendrobium
    Teixeira da Silva, Jaime A.
    Dobranszki, Judit
    Cardoso, Jean Carlos
    Chandler, Stephen F.
    Zeng, Songjun
    PLANT CELL REPORTS, 2016, 35 (03) : 483 - 504
  • [22] Harnessing the CRISPR/Cas9 in filamentous fungi for the production of secondary metabolites
    Karim, Md Dilshad
    Abuhena, Md
    Rahman, Lutfur
    Al Rashid, Jubair
    SYSTEMS MICROBIOLOGY AND BIOMANUFACTURING, 2025,
  • [23] Microcyle Conidiation in Filamentous Fungi
    Jung, Boknam
    Kim, Soyeon
    Lee, Jungkwan
    MYCOBIOLOGY, 2014, 42 (01) : 1 - 5
  • [24] AN ELECTROPORATION-BASED SYSTEM FOR HIGH-EFFICIENCY TRANSFORMATION OF GERMINATED CONIDIA OF FILAMENTOUS FUNGI
    CHAKRABORTY, BN
    PATTERSON, NA
    KAPOOR, M
    CANADIAN JOURNAL OF MICROBIOLOGY, 1991, 37 (11) : 858 - 863
  • [25] Genetic engineering of filamentous fungi - Progress, obstacles and future trends
    Meyer, Vera
    BIOTECHNOLOGY ADVANCES, 2008, 26 (02) : 177 - 185
  • [26] The biology and potential for genetic research of transposable elements in filamentous fungi
    de Lima Favaro, Leia Cecilia
    de Araujo, Welington Luiz
    de Azevedo, Joao Lucio
    Paccola-Meirelles, Luzia Doretto
    GENETICS AND MOLECULAR BIOLOGY, 2005, 28 (04) : 804 - 813
  • [27] A Mad7 System for Genetic Engineering of Filamentous Fungi
    Vanegas, Katherina Garcia
    Rendsvig, Jakob Kraemmer Haar
    Jarczynska, Zofia Dorota
    Cortes, Marcio Vinicius de Carvalho Barros
    van Esch, Abel Peter
    Morera-Gomez, Marti
    Contesini, Fabiano Jares
    Mortensen, Uffe Hasbro
    JOURNAL OF FUNGI, 2023, 9 (01)
  • [28] Genetic transformation of lignin degrading fungi facilitated by Agrobacterium tumefaciens
    Sharma, Krishna K.
    Kuhad, Ramesh C.
    BMC BIOTECHNOLOGY, 2010, 10
  • [29] Current views on endocytosis in filamentous fungi
    Commer, Blake
    Shaw, Brian D.
    MYCOLOGY-AN INTERNATIONAL JOURNAL ON FUNGAL BIOLOGY, 2021, 12 (01) : 1 - 9
  • [30] Status of filamentous fungi in integrated biorefineries
    Troiano, D.
    Orsat, V.
    Dumont, M. J.
    RENEWABLE & SUSTAINABLE ENERGY REVIEWS, 2020, 117 (117)
12345