Methods for genetic transformation of filamentous fungi

被引：129
|
作者：
Li, Dandan [1 ,3 ]
Tang, Yu [4 ]
Lin, Jun [1 ,2 ,3 ]
Cai, Weiwen [1 ,3 ]
机构：
[1] Fuzhou Univ, Inst Apply Genom, 2 Xueyuan Rd, Fuzhou 350108, Fujian, Peoples R China
[2] Fujian Med Univ, Sch Basic Med Sci, 1 Xuefubei Rd, Fuzhou 350122, Fujian, Peoples R China
[3] Fuzhou Univ, Coll Biol Sci & Engn, 2 Xueyuan Rd, Fuzhou 350108, Fujian, Peoples R China
[4] Triplex Int Biosci China Co LTD, Xiamen 361100, Peoples R China
来源：
MICROBIAL CELL FACTORIES | 2017年 / 16卷
基金：
中国国家自然科学基金;
关键词：
Filamentous fungi; Protoplast-mediated transformation; Agrobacterium-mediated transformation; Electroporation; Biolistic method; Shock-wave-mediated transformation; AGROBACTERIUM-MEDIATED TRANSFORMATION; HIGH-EFFICIENCY TRANSFORMATION; ASPERGILLUS-NIDULANS; TRICHODERMA-REESEI; SHOCK-WAVES; ELECTROPORATION; TUMEFACIENS; PLASMID; DNA; SYSTEM;
D O I：
10.1186/s12934-017-0785-7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a precondition that enables scientists to target and modify genes efficiently and may reveal the function of target genes. The method to deliver foreign nucleic acid into cells is the sticking point for fungal genome modification. Up to date, there are some general methods of genetic transformation for fungi, including protoplast-mediated transformation, Agrobacterium-mediated transformation, electroporation, biolistic method and shock-wave-mediated transformation. This article reviews basic protocols and principles of these transformation methods, as well as their advantages and disadvantages.
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页数：13
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