Genetic characterization of Metapenaeus affinis (H. M. Edwards, 1837) using RAPD markers

被引：8

作者：

Lakra, W. S. ^{[1
]}

Goswami, M. ^{[1
]}

Singh, Akhilesh ^{[1
]}

Mishra, Premsagar ^{[2
]}

Gayathri, N. ^{[2
]}

Nagpure, N. S. ^{[1
]}

机构：

[1] Natl Bur Fish Genet Resources, Lucknow 226002, Uttar Pradesh, India

[2] Cent Inst Fisheries Educ, Bombay 400061, Maharashtra, India

来源：

MOLECULAR BIOLOGY REPORTS | 2010年 / 37卷 / 08期

关键词：

Metapenaeus affinis; RAPD; Genetic characterization; INDIAN SCIAENIDS PISCES; SHRIMP PENAEUS-MONODON; 16S RIBOSOMAL-RNA; MOLECULAR-IDENTIFICATION; POPULATIONS; DIVERSITY; PERCIFORMES;

D O I：

10.1007/s11033-010-0029-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetic structure of four populations of Metapenaeus affinis from Maharashtra, Orissa, Kerala and Tamil Nadu in India was studied using RAPD markers. Five selective primers provided distinct and consistent RAPD profiles in all the four populations. The bands in the range 225-1,900 bp were scored for consistent results. The RAPD profiles generated by all the five primers revealed varying degrees of polymorphism, ranging from 25.00% (primer E-03) to 65.00% (primer E-06). Nei's (Nei M, Natl Acad Sci Proc USA 70:3321-3323, 1973) genetic diversity (h) among the four populations varied from 0.2565 +/- A 0.2146 (Orissa population) to 0.3576 +/- A 0.1897 (Maharashtra population).

引用

页码：3757 / 3761

页数：5

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