Activity of covalently immobilised PGA in water miscible solvents at controlled aw

被引：16

作者：

Basso, A ^{[1
]}

De Martin, L ^{[1
]}

Ebert, C ^{[1
]}

Gardossi, L ^{[1
]}

Linda, P ^{[1
]}

Zlatev, V ^{[1
]}

机构：

[1] Univ Trieste, Dipartimento Sci Farmaceut, I-34127 Trieste, Italy

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2001年 / 11卷 / 4-6期

关键词：

water activity; immobilised PGA; water miscible solvents; amide synthesis;

D O I：

10.1016/S1381-1177(00)00070-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Covalently immobilised penicillin G acylase is active in both apolar and water miscible solvents. Hydrated Celite R-640 added to water miscible solvents prevents the medium from stripping the water off the enzyme. The porous siliceous matrix has been used also for the dehydration and storage of the enzyme in apolar media. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：851 / 855

页数：5

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