Serum thyroglobulin stability for immunoassay

被引：6

作者：

Gao, Yun-chao ^{[1
]}

Yang, Ya-dong ^{[1
]}

Yuan, Zhi-bin ^{[1
]}

Lu, Han-kui ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Immunoassay Lab, Dept Nucl Med, Shanghai Peoples Hosp 6, Shanghai 200030, Peoples R China

来源：

LABMEDICINE | 2007年 / 38卷 / 10期

关键词：

D O I：

10.1309/HUBPYXQKU0JADFN2

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: Serum thyroglobulin (Tg) measurements may be affected by preanalytical factors, such as sample handling and processing, which is not quite clear at present. Methods: The authors measured Tg in sera left on the clot (4 degrees C), or timely pipetted off the clot (RT), or= subjected to 5 cycles of freezing (-80 degrees C, 3 months at cycle 4 and 24 h at the other cycles) and thawing (RT 1,h). Result: Sample storage for,4 to 24 h yielded Tg concentrations of 102.4 % to 104.5% (P=0.484) for unseparated, and 98.4 %: to 102.1% (P=0.202) for timely separated sera relative to baseline samples (1 h). Freezing-thawing resulted in significant decreases in Tg concentrations (P < 0.001) by mean 30.7% after the last cycle, with the greatest single cycle decrease by mean 18.1 % at cycle 4. Conclusion: Thyroglobulin. is stable for at least 24 h in unseparated sera at 4 C or separated sera at room temperature, but not in sera undergoing even a short period of frozen storage.

引用

页码：618 / 620

页数：3