Prevalence of crt and mdr-1 mutations in Plasmodium falciparum isolates from Grande Comore island after withdrawal of chloroquine

被引:23
作者
Huang, Bo [1 ]
Wang, Qi [1 ]
Deng, Changsheng [1 ]
Wang, Jianhua [1 ]
Yang, Tao [1 ]
Huang, Shiguang [2 ]
Su, Xin-zhuan [3 ,4 ]
Liu, Yajun [5 ]
Pan, Longhua [6 ]
Li, Guoming [6 ]
Li, Di [6 ]
Zhang, Hongying [1 ]
Bacar, Afane [7 ]
Abdallah, Kamal Said [7 ]
Attoumane, Rachad [7 ]
Mliva, Ahamada M. S. A. [8 ]
Zheng, Shaoqin [1 ]
Xu, Qin [9 ]
Lu, Fangli [10 ]
Guan, Yezhi [1 ]
Song, Jianping [1 ]
机构
[1] Guangzhou Univ Chinese Med, Sci & Technol Pk, Guangzhou 510006, Guangdong, Peoples R China
[2] Jinan Univ, Sch Med, Guangzhou 510632, Guangdong, Peoples R China
[3] NIAID, Lab Malaria & Vector Res, NIH, Bethesda, MD 20892 USA
[4] Xiamen Univ, Sch Life Sci, State Key Lab Cellular Stress Biol, Xiamen 361005, Fujian, Peoples R China
[5] Guangzhou Univ Chinese Med, Affiliated Hosp 1, Guangzhou 510006, Guangdong, Peoples R China
[6] Guangdong Newsouth Artepharm Co Ltd, Guangzhou 510405, Guangdong, Peoples R China
[7] Natl Malaria Control Programme, BP 500 Moroni, Moroni, Comoros
[8] Minist Hlth Comoros, BP 500 Moroni, Moroni, Comoros
[9] Guangzhou Univ Chinese Med, Res Inst Trop Med, Guangzhou 510006, Guangdong, Peoples R China
[10] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510080, Guangdong, Peoples R China
基金
中国博士后科学基金; 美国国家卫生研究院;
关键词
Comoros; Plasmodium falciparum; Chloroquine resistance; pfcrt; pfmdr-1; GENE HAPLOTYPE SVMNT; DRUG-RESISTANCE; ARTEMETHER-LUMEFANTRINE; MOLECULAR MARKER; TRANSPORTER GENE; TREATMENT POLICY; COPY NUMBER; IN-VIVO; MALARIA; PFCRT;
D O I
10.1186/s12936-016-1474-4
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Background: In Comoros, the widespread of chloroquine (CQ)-resistant Plasmodium falciparum populations was a major obstacle to malaria control, which led to the official withdrawal of CQ in 2004. Continuous monitoring of CQ-resistant markers of the P. falciparum CQ resistant transporter (pfcrt) and the P. falciparum multiple drug resistance 1 (pfmdr-1) is necessary inder to obtain first-hand information on CQ susceptibility of parasite populations in the field. The objective of this study is to assess the prevalence and evolution of CQ-resistance in the P. falciparum populations on the Comoros' Grande Comore island after withdrawal of CQ. Methods: A total of 207 P. falciparum clinical isolates were collected from the island, including 118 samples from 2006 to 2007 and 89 samples from 2013 to 2014. Nucleotide substitutions in the pfcrt and pfmdr-1 genes linked to CQ response in parasite isolates were assessed using nested PCR and DNA sequencing. Results: From the pfcrt gene segment sequenced, we detected C72S, M74I, N75E, and K76T substitutions in the parasite isolates collected from both 2006-2007 to 2013-2014 periods. Significant decline of pfcrt resistant alleles at C72S (42.6 to 6.9 %), M74I (39.1 to 14.9 %), N75E (63.5 to 18.3 %), and K76T (72.2 to 19.5 %) from 2006-2007 to 2013-2014 were observed, and the frequency of pfcrt wild type allele was significantly increased from 19.1 % in 2006-2007 to 75.8 % in 2013-2014. Sequence analysis of pfmdr-1 also detected point mutations at codons N86Y, Y184F, and D1246Y, but not S1034C and N1042D, in the isolates collected from both examined periods. An increasing trend in the prevalence of the pfmdr-1 wild type allele (NYD, 4.3 % in 2006-2007; and 28.7 % in 2013-2014), and a decreasing trend for pfmdr-1 N86Y mutation (87.0 % in 2006-2007; and 40.2 % in 2013-2014) were observed in our samples. Conclusions: The present data indicate that the prevalence and patterns of mutant pfcrt and pfmdr-1 dramatically decreased in the Grande Comore isolates from 2006 to 2014, suggesting that the CQ-sensitive P. falciparum strains have returned after the withdrawal of CQ. The data also suggests that the parasites with wild type pfcrt/pfdmr-1 genes may have growth and/or transmission advantages over the mutant parasites. The information obtained from this study will be useful for developing and updating anti-malarial treatment policy in Grande Comore island.
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