Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein

被引：587
作者：
Ma, WJ
Cheng, S
Campbell, C
Wright, A
Furneaux, H
机构：
[1] MEM SLOAN KETTERING CANC CTR, MOLEC NEUROONCOL LAB, PROGRAM MOL PHARMACOL & THERAPEUT, NEW YORK, NY 10021 USA
[2] CLEVELAND CLIN FDN, RES INST, DEPT CANC BIOL, CLEVELAND, OH 44195 USA
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 1996年 / 271卷 / 14期
关键词：
D O I：
10.1074/jbc.271.14.8144
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The neuronal-specific Elav-like proteins (HuD, Hel-N, and HuC) contain three RNP-type concensus motifs and bind to AU-rich elements. We have identified and cloned a fourth member of this family (HuR) that is expressed in a wide variety of cell types. The purified recombinant protein binds avidly to the AU-rich element in c-fos and interleukin-3 mRNAs, In the case of the c-fos AU-rich element, HuR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Mutational analysis has shown that all three AU motifs are required for maximal binding.
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页码：8144 / 8151
页数：8
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