Membrane fluidization triggers membrane remodeling which affects the thermotolerance in Escherichia coli

被引：73
作者：
Shigapova, N
Török, Z
Balogh, G
Goloubinoff, P
Vígh, L
Horváth, I
机构：
[1] Hungarian Acad Sci, Biol Res Ctr, Inst Biochem, H-6701 Szeged, Hungary
[2] Univ Lausanne, Dept Plant Mol Biol, CH-1015 Lausanne, Switzerland
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 328卷 / 04期
基金：
匈牙利科学研究基金会;
关键词：
membrane fluidity; lipid saturation; thermosensor; heat stress; molecular chaperones; acquired thermotolerance;
D O I：
10.1016/j.bbrc.2005.01.081
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Treatment of Escherichia coli with non-lethal doses of heat or benzyl alcohol (BA) causes transient membrane fluidization and permeabilization, and induces the rapid transcription of heat-shock genes in a sigma(32) -dependent manner. This early response is followed by a rapid adaptation (priming) of the cells to otherwise lethal elevated temperature, in strong correlation with an observed remodeling of the composition and alkyl chain unsaturation of membrane lipids. The acquisition of cellular thermotolerance in BA-primed cells is unrelated to protein denaturation and is not accompanied by the formation of major heat-shock proteins, such as GroEL and DnaK. This suggests that the rapid remodeling of membrane cornposition is sufficient for the short-term bacterial thermotolerance. (C) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码：1216 / 1223
页数：8
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