Determination of ibuprofen enantiomers in human plasma by HPLC-MS/MS: validation and application in neonates

被引：20
作者：
Chen, Tingting [1 ]
Li, Qin [1 ]
Lu, Jinmiao [1 ]
Yu, Chen [2 ]
Chen, Chao [3 ]
Li, Zhiping [1 ]
机构：
[1] Fudan Univ, Childrens Hosp, Dept Pharm, 399 Wanyuan Rd, Shanghai 201102, Peoples R China
[2] Shanghai Xuhui Cent Hosp, Cent Lab, Shanghai, Peoples R China
[3] Fudan Univ, Childrens Hosp, Dept Neonatol, Shanghai 201102, Peoples R China
来源：
BIOANALYSIS | 2016年 / 8卷 / 12期
关键词：
enantiomers; HPLC-MS/MS; ibuprofen; interference; neonate; PERFORMANCE LIQUID-CHROMATOGRAPHY; RESOLUTION MASS-SPECTROMETRY; ENANTIOSELECTIVE DETERMINATION; PREMATURE-INFANTS; CHIRAL INVERSION; PRETERM INFANTS; PHARMACOKINETICS; DERIVATIZATION; SERUM;
D O I：
10.4155/bio-2016-0013
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Aim: An adaptive method to determine ibuprofen enantiomers with limited volume of plasma required is necessary for investigating PK of ibuprofen in neonates. Results: Enantiomer separation was achieved on a Lux cellulose 3 column with mobile phase consisting of methanol water (85: 15, v/v) and formic acid (0.0075%) at isocratic rate of 0.2 ml/min. Calibration curve is linear for each enantiomer at the range of 0.1-60 mu g/ml. Validation was conducted and results met requirements regarding to intra-and inter-run precision, accuracy and recovery. No matrix effect or interference was observed from neonatal plasma or comedications. Only 20 mu l of plasma was requested in this study. Conclusion: This assay was specific and reliable to quantify ibuprofen enantiomers in neonate plasma.
引用
收藏
页码：1237 / 1250
页数：14
相关论文
共 50 条
[21]   Determination of risperidone in human plasma by HPLC-MS/MS and its application to a pharmacokinetic study in Chinese volunteers [J].
Mingzhu HUANG Jianzhong SHENTU Junchun CHEN Jian LIU Huili ZHOU Research Center of Clinical Pharmaceutical Science the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou China .
Journal of Zhejiang University(Science B:An International Biomedicine & Biotechnology Journal), 2008, (02) :114-120
[22]   Determination of risperidone in human plasma by HPLC-MS/MS and its application to a pharmacokinetic study in Chinese volunteers [J].
Huang, Ming-Zhu ;
Shentu, Jian-Zhong ;
Chen, Jun-Chun ;
Liu, Jian ;
Zhou, Hui-Li .
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B, 2008, 9 (02) :114-120
[23]   Quantification of hydroxyurea in human plasma by HPLC-MS/MS and its application to pharmacokinetics in patients with chronic myeloid leukaemia [J].
Hai, Xin ;
Guo, Meihua ;
Gao, Chunlu ;
Zhou, Jin .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2017, 137 :213-219
[24]   Development and validation of a HPLC-MS/MS method with electrospray ionization for quantitation of potassium oxonate in human plasma: Application to a pharmacokinetic study [J].
Hao, Guangtao ;
Bai, Shaobo ;
Liang, Haixia ;
Liang, Yuguang ;
Qu, Hengyan ;
Gao, Hongzhi ;
Li, Yuanyuan ;
Zheng, Zhuanjie ;
Wang, Xiaofang ;
Liu, Zeyuan .
EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2013, 5 (03) :932-936
[25]   Development and Validation of an Improved HPLC-MS/MS Method for Quantifying Total and Unbound Lenalidomide in Human Plasma [J].
Lee, Suhyun ;
Yang, Seungwon ;
Shim, Wang-Seob ;
Song, Eunseo ;
Han, Seunghoon ;
Park, Sung-Soo ;
Choi, Suein ;
Joo, Sung Hwan ;
Park, Seok Jun ;
Shin, Beomjin ;
Kim, Donghyun ;
Kim, Hyeonsu ;
Jung, Yujung ;
Lee, Kyung-Tae ;
Chung, Eun Kyoung .
PHARMACEUTICS, 2024, 16 (10)
[26]   Quantitative determination of amisulpride in rat plasma by HPLC-MS/MS [J].
Noh, Keumhan ;
Jang, Yoo-Jeong ;
Kwon, Kwang-il ;
Kim, Eunyoung ;
Jeong, Tae Cheon ;
Yun, Hwi-yeol ;
Kang, Wonku .
ARCHIVES OF PHARMACAL RESEARCH, 2015, 38 (01) :63-67
[27]   Separation and determination of acetyl-glutamine enantiomers by HPLC-MS and its application in pharmacokinetic study [J].
Zhang, Xiaoxiao ;
Gao, Lei ;
Zhang, Zunjian ;
Tian, Yuan .
JOURNAL OF PHARMACEUTICAL ANALYSIS, 2017, 7 (05) :303-308
[28]   Determination of Boc5 in Rat Plasma by HPLC-MS/MS and Its Application to a Pharmacokinetic Study [J].
Li, Ruixing ;
Fan, Huirong ;
Gu, Yuan ;
Si, Duanyun ;
Liang, Wei .
CHROMATOGRAPHIA, 2012, 75 (13-14) :721-727
[29]   HPLC-MS/MS Method Validation for Antifungal Agents in Human Serum in Clinical Application [J].
Choi, Hyebin ;
Yim, Jisook ;
Cha, Jae Hyun ;
Kim, Juyeon ;
Kim, Keun Ju ;
Nam, Minjeong ;
Nam, Myung Hyun ;
Lee, Chang Kyu ;
Cho, Yunjung ;
Yun, Seung Gyu .
CLINICAL LABORATORY, 2023, 69 (09) :1916-1923
[30]   Development and validation of a method for the determination of a therapeutic peptide with affinity for the human B1 receptor in human plasma using HPLC-MS/MS [J].
Wilson, S. F. ;
Li, H. ;
Rose, M. J. ;
Xiao, J. ;
Holder, J. R. ;
James, C. A. .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2010, 878 (9-10) :749-757
12345