Chemiluminescence immunoassay in microemulsions

被引：13

作者：

Kamyshny, A ^{[1
]}

Magdassi, S ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Casali Inst Appl Chem, IL-91904 Jerusalem, Israel

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 1998年 / 11卷 / 05期

关键词：

chemiluminescence; microemulsions; immunoassay; adsorption; IgG; microparticles;

D O I：

10.1016/S0927-7765(98)00044-7

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A novel chemiluminescence immunoassay which combines microparticles and microemulsion is described. The method is based on a specific interaction of an antibody with an antigen preadsorbed onto fluorescer (perylene) microparticles (mean diameter 0.8-1.6 mu m). Dissolving the particles in a microemulsion-forming mixture containing bis(2,4,6-trichlorophenyl) oxalate in toluene (oil phase) and H2O2 in Triton X-100 (surfactant)/2-butanol (cosurfactant)/water leads to chemiluminescence. The dependence of the chemiluminescence intensity on the concentrations of antigen, antibody and fluorescer, as well as the kinetics of the chemiluminescence decay, were studied. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：249 / 254

页数：6

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