A new HPTLC densitometric method for analysis of swertiamarin in Enicostemma littorale and commercial formulations

被引：16

作者：

Alam, P. ^{[1
,2
]}

Ali, M. ^{[2
]}

Singh, R. ^{[3
]}

Shakeel, F. ^{[4
]}

机构：

[1] Al Kharj Univ, Coll Pharm, Dept Pharmacognosy, Al Kharj, Saudi Arabia

[2] Jamia Hamdard, Dept Pharmacognosy & Phytochem, New Delhi 110062, India

[3] Ranbaxy Res Labs, Herbal Drug Res Analyt Dev, Gurgaon, Haryana, India

[4] Al Arab Med Univ, Dept Pharmaceut, Benghazi, Libya

来源：

NATURAL PRODUCT RESEARCH | 2011年 / 25卷 / 01期

关键词：

swertiamarin; HPTLC densitometry; ICH guidelines; Enicostemma littorale; EXTRACT; BLUME;

D O I：

10.1080/14786411003754348

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A high-performance thin layer chromatographic densitometric method for the analysis of swertiamarin in 60% methanolic extract of Enicostemma littorale and commercial formulations has been developed and validated in this study. Swertiamarin was separated on aluminium-backed silica gel 60 F-254 plates using ethyl acetate : methanol : water ( 77 : 15 : 8)% v/v as the mobile phase. This system was found to give a compact spot of swertiamarin at R-f value 0.36 +/- 0.01. The limit of detection and limit of quantification were found to be 31.25 and 103.12 ng spot(-1), respectively. The proposed method was employed with a high degree of precision and accuracy for the estimation of swertiamarin in methanolic extract of Enicostemma littorale and in commercial formulations.

引用

页码：17 / 25

页数：9

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