A kinetic model for the penicillin biosynthetic pathway in Penicillium chrysogenum

被引：11

作者：

Nielsen, J

Jorgensen, HS

机构：

[1] Center for Process Biotechnology, Department of Biotechnology, Technical University of Denmark

来源：

CONTROL ENGINEERING PRACTICE | 1996年 / 4卷 / 06期

关键词：

kinetic model; Penicillium chrysogenum; metabolic control analysis; penicillin; metabolic engineering; ACV synthetase; isopenicillin N synthetase;

D O I：

10.1016/0967-0661(96)00067-6

中图分类号：

TP [自动化技术、计算机技术];

学科分类号：

0812 ;

摘要：

A kinetic model for the first two steps in the penicillin biosynthetic pathway, i.e. the ACV synthetase (ACVS) and the isopenicillin N synthetase (IPNS) is proposed. The model is based on Michaelis-Menten type kinetics with non-competitive inhibition of the ACVS by ACV, and competitive inhibition of the IPNS by glutathione. The model predicted flux through the pathway corresponds well with the measured rate of penicillin biosynthesis. From the kinetic model the elasticity coefficients and the flux control coefficients are calculated throughout a fed-batch cultivation, and it is found that there is a shift in the flux control from the ACVS to the IPNS during the cultivation.

引用

页码：765 / 771

页数：7

共 25 条

[1] THE ISOPENICILLIN-N ACYLTRANSFERASE OF PENICILLIUM-CHRYSOGENUM HAS ISOPENICILLIN-N AMIDOHYDROLASE, 6-AMINOPENICILLANIC ACID ACYLTRANSFERASE AND PENICILLIN AMIDASE ACTIVITIES, ALL OF WHICH ARE ENCODED BY THE SINGLE PENDE GENE [J].