CsGOGAT Is Important in Dynamic Changes of Theanine Content in Postharvest Tea Plant Leaves under Different Temperature and Shading Spreadings

被引：40

作者：

Liu, Zhi-Wei ^{[1
]}

Li, Hui ^{[1
]}

Wang, Wen -Li ^{[1
]}

Wu, Zhi-Jun ^{[1
]}

Cui, Xin ^{[1
]}

Zhuang, Jing ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Hort, Tea Sci Res Inst, Nanjing 210095, Jiangsu, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2017年 / 65卷 / 44期

基金：

中国国家自然科学基金;

关键词：

postharvest leaves; spreading; differentially expressed protein; gene expression; CsGOGAT protein; protein-protein interaction; tea plant; FREE AMINO-ACIDS; DEPENDENT GLUTAMATE SYNTHASE; GENE-EXPRESSION; BIOSYNTHESIS; ENVIRONMENT; PHASE;

D O I：

10.1021/acs.jafc.7b04552

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

We analyzed the changes of theanine content in postharvest tea leaves under high temperature (38 degrees C), low temperature (4 degrees C), and shading spreadings by using ultrahigh-performance liquid chromatography. The differentially expressed proteins (DEPs), CsFd-GOGAT and CsNADH-GOGAT, which are involved in theanine biosynthesis pathway, were identified from the corresponding proteome data. The protein protein interactions of CsFd-GOGAT and CsNADH-GOGAT, CsTS1, or CsNiR were verified by yeast two-hybrid technology. The expression profiles of 17 genes in theanine metabolism, including CsFd-GOGAT and CsNADH-GOGAT, were analyzed by quantitative real-time polymerase chain reaction. The correlations between the dynamic changes of theanine content and expression profiles of related genes and DEPs were analyzed. This study preliminarily proved the importance of CsGOGAT in dynamic changes of theanine content in postharvest tea leaves during spreading.

引用

页码：9693 / 9702

页数：10

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