Diphenyleneiodonium inhibits the activation of mitogen-activated protein kinases and the expression of monocyte chemoattractant protein-1 in Helicobacter pylori-infected gastric epithelial AGS cells

被引：7
作者：
Cho, Soon Ok [2 ]
Lim, Joo Weon [1 ]
Kim, Kyung Hwan [2 ]
Kim, Hyeyoung [1 ]
机构：
[1] Yonsei Univ, Coll Human Ecol, Dept Food & Nutr, Brain Korea Project 21, Seoul 120749, South Korea
[2] Yonsei Univ, Coll Med, Dept Pharmacol, Brain Korea Project Med Sci 21, Seoul 120752, South Korea
来源：
INFLAMMATION RESEARCH | 2011年 / 60卷 / 05期
关键词：
Helicobacter pylori; NADPH oxidase; Monocyte chemoattractant protein-1; Mitogen-activated protein kinase; Gastric epithelial cells; NF-KAPPA-B; NADPH OXIDASE; CHEMOKINE EXPRESSION; ENDOTHELIAL-CELLS; CANCER; CYCLOOXYGENASE-2; TRANSCRIPTION; APOPTOSIS; CYTOKINES; PATHWAYS;
D O I：
10.1007/s00011-010-0297-y
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
To investigate whether NADPH oxidase induces MCP-1 expression and the activation of mitogen-activated protein kinases (MAPKs) in H. pylori-infected gastric epithelial cells. H. pylori in Korean isolates, human gastric epithelial AGS cells AGS cells pretreated with or without an NADPH oxidase inhibitor diphenyleneiodonium (DPI) are cultured in the presence of H. pylori at a bacterium/cell ratio of 300:1. Reactive oxygen species (ROS) and MCP-1 were determined by confocal microscopy and enzyme-linked immonosorbent assay. NADPH oxidase activity was measured by lucigenin assay. mRNA expression of MCP-1 was analyzed by reverse transcription-polymerase chain reaction. Levels of MAPKs were assessed by Western blot analysis. H. pylori induced increase in ROS, NADPH oxidase activity, MCP-1 expression, and the activation of MAPKs including extracellular signal-regulated kinases, p38, and jun N-terminal kinases in AGS cells, which was inhibited by DPI. Inhibiting NADPH oxidase by DPI suppresses H. pylori-induced activation of MAPKs and MCP-1 expression in AGS cells.
引用
收藏
页码：501 / 507
页数：7
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