Purified Auricularia auricular-judae polysaccharide (AAP I-a) prevents oxidative stress in an ageing mouse model

被引：88
作者：
Zhang, Hua [1 ]
Wang, Zhen-Yu [1 ,2 ]
Zhang, Zhi [2 ]
Wang, Xue [3 ]
机构：
[1] Harbin Inst Technol, Sch Food Sci & Engn, Harbin 150090, Peoples R China
[2] NE Forestry Univ, Sch Forestry, Harbin 150040, Peoples R China
[3] Symrise Shanghai Co Ltd, Shanghai 201206, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2011年 / 84卷 / 01期
关键词：
Auricularia auricula-judae; Polysaccharide; Oxidative stress; Ageing mouse model; BETA-D-GLUCAN; LYCIUM-BARBARUM POLYSACCHARIDES; BLOOD LIPID-METABOLISM; ANTIOXIDANT ACTIVITY; ANTITUMOR-ACTIVITY; ANTICOAGULANT ACTIVITY; DAMAGE; VITRO; MICE; VIVO;
D O I：
10.1016/j.carbpol.2010.12.044
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
This paper reports on a water-soluble polysaccharide (AAP I-a) extracted from Auricularia auricular with assistance from ultrasonics, and purified by anion-exchange and gel-permeation chromatography. Additional further structural characteristics were determined from high-performance liquid chromatography/gel permeation chromatograph (HPLC/GPC), Fourier transform infrared (FT-IR) spectrometer and gas chromatography-mass spectrophotometer (GC-MS). AAP I-a is composed of L-rhamnose, L-arabinose, D-xylose, D-mannose, D-glucose, and D-galactose in a molar ratio of 0.2:2.6:0.4:3.6:1.0:0.4. After 35 days of AAP I-a oral administration (50, 100 and 200 mg/kg once a day), the AAP I-a significantly decreased the level of malondialdehyde (MDA) and increased superoxide dismutase (SOD) and glutathione (GSH) activities in mice where ageing is induced by D-galactose (p < 0.05). In conclusion, our results indicated that AAP I-a possessed potent antioxidant activity. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：638 / 648
页数：11
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